Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   SAPIG_RS08375 Genome accession   NC_017333
Coordinates   1691510..1692007 (-) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus subsp. aureus ST398     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1686510..1697007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAPIG_RS08350 (SAPIG1600) gcvPB 1686672..1688144 (-) 1473 WP_000202179.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  SAPIG_RS08355 (SAPIG1601) gcvPA 1688137..1689483 (-) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  SAPIG_RS08360 (SAPIG1602) gcvT 1689503..1690594 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  SAPIG_RS08365 (SAPIG1603) - 1690753..1691277 (-) 525 WP_001015120.1 shikimate kinase -
  SAPIG_RS08370 - 1691267..1691413 (-) 147 WP_001789879.1 hypothetical protein -
  SAPIG_RS08375 (SAPIG1604) comGF 1691510..1692007 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  SAPIG_RS08380 (SAPIG1605) comGE 1691925..1692224 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  SAPIG_RS08385 (SAPIG1606) comGD 1692211..1692657 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  SAPIG_RS08390 (SAPIG1607) comGC 1692635..1692946 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  SAPIG_RS08395 (SAPIG1608) comGB 1692960..1694030 (-) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  SAPIG_RS08400 (SAPIG1609) comGA 1694002..1694976 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  SAPIG_RS08405 (SAPIG1610) - 1695028..1695651 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  SAPIG_RS08410 (SAPIG1611) - 1695648..1695977 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SAPIG_RS08415 (SAPIG1612) - 1695977..1696963 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=46249 SAPIG_RS08375 WP_001825340.1 1691510..1692007(-) (comGF) [Staphylococcus aureus subsp. aureus ST398]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=46249 SAPIG_RS08375 WP_001825340.1 1691510..1692007(-) (comGF) [Staphylococcus aureus subsp. aureus ST398]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97

Multiple sequence alignment