Detailed information NTG0459556

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HV227_RS22510
• Genome accession: NZ_CP056425
• Coordinates: 4470902..4471702 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain RHBSTW-00515
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4465902..4476702

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV227_RS22475 (HV227_22475)	gspH	4466058..4466573 (+)	516	WP_004194939.1	type II secretion system minor pseudopilin GspH	-
HV227_RS22480 (HV227_22480)	gspI	4466570..4466935 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
HV227_RS22485 (HV227_22485)	gspJ	4466932..4467528 (+)	597	WP_004191953.1	type II secretion system minor pseudopilin GspJ	-
HV227_RS22490 (HV227_22490)	gspK	4467525..4468511 (+)	987	WP_021467522.1	type II secretion system minor pseudopilin GspK	-
HV227_RS22495 (HV227_22495)	gspL	4468498..4469691 (+)	1194	WP_048265188.1	type II secretion system protein GspL	-
HV227_RS22500 (HV227_22500)	-	4469684..4470169 (+)	486	WP_004145896.1	type II secretion system protein M	-
HV227_RS22505 (HV227_22505)	-	4470159..4470905 (+)	747	WP_048265187.1	type II secretion system protein N	-
HV227_RS22510 (HV227_22510)	pilD	4470902..4471702 (+)	801	WP_004191960.1	prepilin peptidase	Machinery gene
HV227_RS22515 (HV227_22515)	hrpB	4471750..4474179 (-)	2430	WP_048265186.1	ATP-dependent helicase HrpB	-
HV227_RS22520 (HV227_22520)	thpR	4474252..4474788 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
HV227_RS22525 (HV227_22525)	sfsA	4474788..4475504 (+)	717	WP_048265185.1	DNA/RNA nuclease SfsA	-
HV227_RS22530 (HV227_22530)	dksA	4475667..4476122 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=459556 HV227_RS22510 WP_004191960.1 4470902..4471702(+) (pilD) [Klebsiella pneumoniae strain RHBSTW-00515]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=459556 HV227_RS22510 WP_004191960.1 4470902..4471702(+) (pilD) [Klebsiella pneumoniae strain RHBSTW-00515]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCACAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421