Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   JHW37_RS24435 Genome accession   NZ_CP068238
Coordinates   5209567..5209992 (+) Length   141 a.a.
NCBI ID   WP_034081452.1    Uniprot ID   E9KTN5
Organism   Pseudomonas aeruginosa strain A39-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5204567..5214992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW37_RS24420 (JHW37_24420) pilX 5205155..5205742 (+) 588 WP_034081449.1 type 4a pilus minor pilin PilX -
  JHW37_RS24425 (JHW37_24425) pilY1 5205754..5209221 (+) 3468 WP_053817771.1 type 4a pilus biogenesis protein PilY1 -
  JHW37_RS24430 (JHW37_24430) pilY2 5209223..5209570 (+) 348 WP_043101203.1 type 4a fimbrial biogenesis protein PilY2 -
  JHW37_RS24435 (JHW37_24435) comF 5209567..5209992 (+) 426 WP_034081452.1 type IV pilin protein Machinery gene
  JHW37_RS24440 (JHW37_24440) ispH 5210041..5210985 (-) 945 WP_034081453.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  JHW37_RS24445 (JHW37_24445) fkpB 5211071..5211511 (-) 441 WP_003150012.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  JHW37_RS24450 (JHW37_24450) lspA 5211504..5212013 (-) 510 WP_034081454.1 signal peptidase II -
  JHW37_RS24455 (JHW37_24455) ileS 5212006..5214837 (-) 2832 WP_053814570.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15256.32 Da        Isoelectric Point: 9.3371

>NTDB_id=459262 JHW37_RS24435 WP_034081452.1 5209567..5209992(+) (comF) [Pseudomonas aeruginosa strain A39-1]
MTKKEKGFTLLEMMIVVVLIGILLGIAIPSYQNYVIRANRTEGQALLSDAAARQERYYSQNPGVGYTNDVAKLGMSSANS
PNNLYSLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNDSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=459262 JHW37_RS24435 WP_034081452.1 5209567..5209992(+) (comF) [Pseudomonas aeruginosa strain A39-1]
ATGACGAAAAAAGAGAAAGGCTTCACCTTGCTGGAGATGATGATCGTCGTGGTGCTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCGCCAACCGCACCGAAGGCCAGGCGCTGCTCTCGGACGCGGCGGCACGCC
AGGAGCGCTACTATTCGCAGAACCCCGGGGTCGGTTACACCAACGACGTGGCCAAGCTGGGCATGAGCTCGGCCAACTCG
CCAAACAACCTGTACAGCCTCACCATAGCGACGCCCACCAGTACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACTCTCAACCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGTAACGACA
GCACGGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E9KTN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.52

90.071

0.383
  pilE Legionella pneumophila str. Paris

38.571

99.291

0.383
  pilE Legionella pneumophila strain ERS1305867

38.571

99.291

0.383