Detailed information NTG0459115

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HV294_RS20820
• Genome accession: NZ_CP056301
• Coordinates: 4293551..4294351 (+)
• Length: 266 a.a.
• NCBI ID: WP_032414527.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain RHBSTW-00843
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4288551..4299351

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV294_RS20785 (HV294_20785)	gspH	4288707..4289222 (+)	516	WP_181467270.1	type II secretion system minor pseudopilin GspH	-
HV294_RS20790 (HV294_20790)	gspI	4289219..4289584 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HV294_RS20795 (HV294_20795)	gspJ	4289581..4290177 (+)	597	WP_009309266.1	type II secretion system minor pseudopilin GspJ	-
HV294_RS20800 (HV294_20800)	gspK	4290174..4291160 (+)	987	WP_115244750.1	type II secretion system minor pseudopilin GspK	-
HV294_RS20805 (HV294_20805)	gspL	4291147..4292340 (+)	1194	WP_181467271.1	type II secretion system protein GspL	-
HV294_RS20810 (HV294_20810)	-	4292333..4292818 (+)	486	WP_004151943.1	type II secretion system protein M	-
HV294_RS20815 (HV294_20815)	-	4292808..4293554 (+)	747	WP_004145897.1	type II secretion system protein N	-
HV294_RS20820 (HV294_20820)	pilD	4293551..4294351 (+)	801	WP_032414527.1	A24 family peptidase	Machinery gene
HV294_RS20825 (HV294_20825)	hrpB	4294399..4296828 (-)	2430	WP_031588872.1	ATP-dependent helicase HrpB	-
HV294_RS20830 (HV294_20830)	thpR	4296901..4297437 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
HV294_RS20835 (HV294_20835)	sfsA	4297437..4298153 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HV294_RS20840 (HV294_20840)	dksA	4298316..4298771 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=459115 HV294_RS20820 WP_032414527.1 4293551..4294351(+) (pilD) [Klebsiella pneumoniae strain RHBSTW-00843]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=459115 HV294_RS20820 WP_032414527.1 4293551..4294351(+) (pilD) [Klebsiella pneumoniae strain RHBSTW-00843]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGTATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCAGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACTCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421