Detailed information NTG0458723

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HV343_RS20855
• Genome accession: NZ_CP056216
• Coordinates: 4255060..4255860 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain RHBSTW-00982
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4250060..4260860

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV343_RS20820 (HV343_20815)	gspH	4250216..4250731 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
HV343_RS20825 (HV343_20820)	gspI	4250728..4251093 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HV343_RS20830 (HV343_20825)	gspJ	4251090..4251686 (+)	597	WP_023279276.1	type II secretion system minor pseudopilin GspJ	-
HV343_RS20835 (HV343_20830)	gspK	4251683..4252669 (+)	987	WP_023279277.1	type II secretion system minor pseudopilin GspK	-
HV343_RS20840 (HV343_20835)	gspL	4252656..4253849 (+)	1194	WP_021441216.1	type II secretion system protein GspL	-
HV343_RS20845 (HV343_20840)	-	4253842..4254327 (+)	486	WP_004151943.1	type II secretion system protein M	-
HV343_RS20850 (HV343_20845)	-	4254317..4255063 (+)	747	WP_004145897.1	type II secretion system protein N	-
HV343_RS20855 (HV343_20850)	pilD	4255060..4255860 (+)	801	WP_004145898.1	A24 family peptidase	Machinery gene
HV343_RS20860 (HV343_20855)	hrpB	4255908..4258337 (-)	2430	WP_023300747.1	ATP-dependent helicase HrpB	-
HV343_RS20865 (HV343_20860)	thpR	4258410..4258946 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
HV343_RS20870 (HV343_20865)	sfsA	4258946..4259662 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HV343_RS20875 (HV343_20870)	dksA	4259825..4260280 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=458723 HV343_RS20855 WP_004145898.1 4255060..4255860(+) (pilD) [Klebsiella pneumoniae strain RHBSTW-00982]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=458723 HV343_RS20855 WP_004145898.1 4255060..4255860(+) (pilD) [Klebsiella pneumoniae strain RHBSTW-00982]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425