Detailed information NTG0458387

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HV324_RS22585
• Genome accession: NZ_CP056136
• Coordinates: 4733029..4733826 (+)
• Length: 265 a.a.
• NCBI ID: WP_086530433.1
• Uniprot ID: -
• Organism: Klebsiella michiganensis strain RHBSTW-00909
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4728029..4738826

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV324_RS22550 (HV324_22555)	gspH	4728170..4728682 (+)	513	WP_046877779.1	type II secretion system minor pseudopilin GspH	-
HV324_RS22555 (HV324_22560)	gspI	4728679..4729044 (+)	366	WP_128349227.1	type II secretion system minor pseudopilin GspI	-
HV324_RS22560 (HV324_22565)	gspJ	4729041..4729637 (+)	597	WP_047724421.1	type II secretion system minor pseudopilin GspJ	-
HV324_RS22565 (HV324_22570)	gspK	4729634..4730614 (+)	981	WP_109212803.1	type II secretion system minor pseudopilin GspK	-
HV324_RS22570 (HV324_22575)	gspL	4730607..4731800 (+)	1194	WP_128349228.1	type II secretion system protein GspL	-
HV324_RS22575 (HV324_22580)	gspM	4731793..4732278 (+)	486	WP_181507158.1	type II secretion system protein GspM	-
HV324_RS22580 (HV324_22585)	-	4732268..4733026 (+)	759	WP_128349229.1	type II secretion system protein N	-
HV324_RS22585 (HV324_22590)	pilD	4733029..4733826 (+)	798	WP_086530433.1	A24 family peptidase	Machinery gene
HV324_RS22590 (HV324_22595)	hrpB	4733924..4736353 (-)	2430	WP_128349230.1	ATP-dependent helicase HrpB	-
HV324_RS22595 (HV324_22600)	thpR	4736426..4736962 (+)	537	WP_049073914.1	RNA 2',3'-cyclic phosphodiesterase	-
HV324_RS22600 (HV324_22605)	sfsA	4736962..4737678 (+)	717	WP_004098690.1	DNA/RNA nuclease SfsA	-
HV324_RS22605 (HV324_22610)	dksA	4737843..4738298 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29064.69 Da
• Isoelectric Point: 7.5684

>NTDB_id=458387 HV324_RS22585 WP_086530433.1 4733029..4733826(+) (pilD) [Klebsiella michiganensis strain RHBSTW-00909]
METIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLFL
KGRSRCCGEPISPRYPLMELATGALFMLVGYLIPPGIPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=458387 HV324_RS22585 WP_086530433.1 4733029..4733826(+) (pilD) [Klebsiella michiganensis strain RHBSTW-00909]
ATGGAAACTATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCACTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGTTTCCTGTTTCTT
AAGGGACGTTCGCGCTGCTGCGGGGAACCCATCTCGCCTCGTTATCCTCTGATGGAGCTGGCGACCGGCGCGCTGTTTAT
GCTGGTCGGATACCTGATACCTCCGGGCATACCGCTGCTGGGCGGGCTAATTTTCGTATCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCTGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAAACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.708	100	0.498
pilD	Neisseria gonorrhoeae MS11	47.431	95.472	0.453
pilD	Vibrio campbellii strain DS40M4	43.273	100	0.449
pilD	Acinetobacter nosocomialis M2	45.174	97.736	0.442
pilD	Acinetobacter baumannii D1279779	44.788	97.736	0.438