Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HV560_RS04765 Genome accession   NZ_CP055305
Coordinates   966397..966951 (+) Length   184 a.a.
NCBI ID   WP_159629170.1    Uniprot ID   A0A7H8UPD7
Organism   Mannheimia pernigra strain 17CN0883     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 961397..971951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV560_RS04740 (HV560_04740) - 961425..962285 (+) 861 WP_159629165.1 metal ABC transporter permease -
  HV560_RS04745 (HV560_04745) - 962278..963102 (+) 825 WP_176807951.1 metal ABC transporter permease -
  HV560_RS04750 (HV560_04750) - 963176..963919 (-) 744 WP_176812267.1 metal ABC transporter ATP-binding protein -
  HV560_RS04755 (HV560_04755) - 963927..964826 (-) 900 WP_176807953.1 metal ABC transporter substrate-binding protein -
  HV560_RS04760 (HV560_04760) - 965080..966240 (+) 1161 WP_159629169.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HV560_RS04765 (HV560_04765) pilF 966397..966951 (+) 555 WP_159629170.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HV560_RS04770 (HV560_04770) - 966952..969288 (+) 2337 WP_176812268.1 Tex family protein -
  HV560_RS04775 (HV560_04775) - 969329..970150 (-) 822 WP_176812269.1 SDR family NAD(P)-dependent oxidoreductase -
  HV560_RS04780 (HV560_04780) - 970152..970715 (-) 564 WP_176812270.1 nitroreductase family protein -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 21095.05 Da        Isoelectric Point: 8.4270

>NTDB_id=457499 HV560_RS04765 WP_159629170.1 966397..966951(+) (pilF) [Mannheimia pernigra strain 17CN0883]
MFAKFFSKITACIILFLLAACSSRFQPSSDAEFNRSEAVKARINLALAYLEQSNFPKAKQNIDKALIHDEKDYLPYSVLA
YYYQKTGDTENAEKSYQTALNLSKNRPDVLNNYGTFLCKQGQFQQAYHQFEQAVESEAPYYHQADTLENIILCAKKEPNL
NKITETLTKLEKLDKQRAILLKGK

Nucleotide


Download         Length: 555 bp        

>NTDB_id=457499 HV560_RS04765 WP_159629170.1 966397..966951(+) (pilF) [Mannheimia pernigra strain 17CN0883]
ATGTTTGCAAAATTTTTCTCAAAAATAACCGCTTGTATAATACTATTTTTATTAGCAGCTTGTTCTTCTCGCTTTCAGCC
TTCCTCTGACGCAGAATTTAATCGATCGGAAGCGGTGAAAGCTCGCATTAATCTTGCTTTGGCTTATTTAGAGCAGAGCA
ATTTCCCCAAAGCGAAACAAAATATTGATAAAGCCCTGATTCACGATGAAAAGGATTATCTACCTTATTCGGTATTGGCG
TACTATTATCAAAAAACAGGGGATACTGAAAATGCTGAAAAATCCTATCAAACTGCCCTAAATTTAAGTAAAAATCGACC
TGATGTTTTAAATAATTACGGTACTTTTTTATGTAAGCAAGGTCAATTTCAGCAAGCTTATCATCAATTTGAACAAGCAG
TTGAAAGCGAAGCACCTTATTATCATCAGGCAGATACTTTAGAAAACATCATTTTATGTGCGAAAAAAGAGCCGAATCTG
AATAAAATAACGGAAACCTTAACTAAGCTCGAAAAATTAGACAAACAACGAGCAATTTTATTGAAAGGAAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(96-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8UPD7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

62.5

100

0.625
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.953

92.935

0.446