Detailed information NTG0457435

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HWA76_RS21015
• Genome accession: NZ_CP055293
• Coordinates: 4281576..4282376 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain 200
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4276576..4287376

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HWA76_RS20980 (HWA76_20945)	gspH	4276732..4277247 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
HWA76_RS20985 (HWA76_20950)	gspI	4277244..4277609 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HWA76_RS20990 (HWA76_20955)	gspJ	4277606..4278202 (+)	597	WP_004191953.1	type II secretion system minor pseudopilin GspJ	-
HWA76_RS20995 (HWA76_20960)	gspK	4278199..4279185 (+)	987	WP_023307453.1	type II secretion system minor pseudopilin GspK	-
HWA76_RS21000 (HWA76_20965)	gspL	4279172..4280365 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
HWA76_RS21005 (HWA76_20970)	-	4280358..4280843 (+)	486	WP_023307455.1	type II secretion system protein M	-
HWA76_RS21010 (HWA76_20975)	-	4280833..4281579 (+)	747	WP_004145897.1	type II secretion system protein N	-
HWA76_RS21015 (HWA76_20980)	pilD	4281576..4282376 (+)	801	WP_004191960.1	prepilin peptidase	Machinery gene
HWA76_RS21020 (HWA76_20985)	hrpB	4282424..4284853 (-)	2430	WP_004191962.1	ATP-dependent helicase HrpB	-
HWA76_RS21025 (HWA76_20990)	thpR	4284926..4285462 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
HWA76_RS21030 (HWA76_20995)	sfsA	4285462..4286178 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HWA76_RS21035 (HWA76_21000)	dksA	4286341..4286796 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=457435 HWA76_RS21015 WP_004191960.1 4281576..4282376(+) (pilD) [Klebsiella pneumoniae strain 200]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=457435 HWA76_RS21015 WP_004191960.1 4281576..4282376(+) (pilD) [Klebsiella pneumoniae strain 200]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421