Detailed information NTG0456860

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HUZ43_RS22495
• Genome accession: NZ_CP055216
• Coordinates: 4701987..4702784 (+)
• Length: 265 a.a.
• NCBI ID: WP_239613811.1
• Uniprot ID: -
• Organism: Raoultella ornithinolytica strain STEFF_15
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4696987..4707784

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUZ43_RS22460 (HUZ43_22355)	gspH	4697107..4697625 (+)	519	WP_015585120.1	type II secretion system minor pseudopilin GspH	-
HUZ43_RS22465 (HUZ43_22360)	gspI	4697622..4697987 (+)	366	WP_004858044.1	type II secretion system minor pseudopilin GspI	-
HUZ43_RS22470 (HUZ43_22365)	gspJ	4697999..4698583 (+)	585	WP_015585121.1	type II secretion system minor pseudopilin GspJ	-
HUZ43_RS22475 (HUZ43_22370)	gspK	4698580..4699566 (+)	987	WP_239613806.1	type II secretion system minor pseudopilin GspK	-
HUZ43_RS22480 (HUZ43_22375)	gspL	4699553..4700755 (+)	1203	WP_088911852.1	type II secretion system protein GspL	-
HUZ43_RS22485 (HUZ43_22380)	gspM	4700748..4701242 (+)	495	WP_044348321.1	type II secretion system protein GspM	-
HUZ43_RS22490 (HUZ43_22385)	-	4701232..4701972 (+)	741	WP_239613808.1	type II secretion system protein N	-
HUZ43_RS22495 (HUZ43_22390)	pilD	4701987..4702784 (+)	798	WP_239613811.1	prepilin peptidase	Machinery gene
HUZ43_RS22500 (HUZ43_22395)	hrpB	4702805..4705234 (-)	2430	WP_239613813.1	ATP-dependent helicase HrpB	-
HUZ43_RS22505 (HUZ43_22400)	thpR	4705307..4705843 (+)	537	WP_004858022.1	RNA 2',3'-cyclic phosphodiesterase	-
HUZ43_RS22510 (HUZ43_22405)	sfsA	4705843..4706559 (+)	717	WP_004858020.1	DNA/RNA nuclease SfsA	-
HUZ43_RS22515 (HUZ43_22410)	dksA	4706724..4707179 (+)	456	WP_004858018.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29346.04 Da
• Isoelectric Point: 8.4762

>NTDB_id=456860 HUZ43_RS22495 WP_239613811.1 4701987..4702784(+) (pilD) [Raoultella ornithinolytica strain STEFF_15]
MTLLSAFSLQFPLLWGGLLFIFGLTFGSFFNVVIYRLPLMMQQEESARFNLCVPASFCPQCQRPIIWRDNIPLLSYLSLK
GRARCCQAPISQRYPLTELASGLLFVLTGYLLTPGLPLLGGLILLSTLLVLAIIDGQTQLLPDRLTLPLLWAGLLFNLNG
TFVPLSEAVIGAMTGYLSLWTVYWLFRLLTGKEALGYGDFKLLAALGAWSGWQILPQTLLCASASGLIWTLLQRRITLQS
LDQPLAFGPWLALAGSGLFLWTQML

Nucleotide

• Length: 798 bp

>NTDB_id=456860 HUZ43_RS22495 WP_239613811.1 4701987..4702784(+) (pilD) [Raoultella ornithinolytica strain STEFF_15]
ATGACGCTACTGAGCGCATTTTCGTTGCAGTTCCCCCTGTTATGGGGCGGATTGTTGTTTATTTTCGGCCTGACGTTTGG
CAGCTTTTTTAACGTGGTTATCTACCGTTTGCCGCTGATGATGCAGCAGGAAGAGTCCGCACGATTCAACCTGTGCGTTC
CCGCCTCCTTCTGCCCCCAGTGCCAGCGGCCCATCATCTGGCGGGATAATATTCCGCTGCTCAGCTATCTCTCACTCAAA
GGGCGCGCCCGCTGCTGCCAGGCGCCCATTTCTCAGCGCTATCCGCTGACGGAACTGGCCAGCGGACTGCTCTTTGTGTT
GACCGGCTATCTGCTGACGCCGGGATTACCGCTGCTGGGGGGCCTGATCCTGCTCTCCACGCTGCTGGTACTGGCGATCA
TCGACGGCCAGACTCAGCTCCTGCCGGATCGGCTGACTCTGCCGCTGCTGTGGGCCGGTCTGCTGTTTAATCTCAACGGC
ACGTTTGTCCCGCTCAGCGAAGCGGTGATCGGCGCCATGACGGGCTATTTATCACTGTGGACGGTTTACTGGCTGTTTCG
CCTGCTGACCGGCAAAGAGGCGCTAGGATACGGCGATTTTAAGCTGCTGGCGGCCCTCGGCGCATGGTCCGGCTGGCAGA
TCCTGCCGCAGACGCTGCTGTGCGCCTCGGCCAGCGGCCTGATCTGGACCTTGCTGCAACGGCGGATCACGCTCCAGTCG
CTGGATCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCAGCGGCCTGTTTCTGTGGACTCAGATGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (122-230)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.254	100	0.498
pilD	Vibrio campbellii strain DS40M4	45.221	100	0.464
pilD	Neisseria gonorrhoeae MS11	46.614	94.717	0.442
pilD	Acinetobacter baumannii D1279779	45.276	95.849	0.434
pilD	Acinetobacter nosocomialis M2	44.882	95.849	0.43