Detailed information NTG0456355

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HUZ80_RS20335
• Genome accession: NZ_CP055119
• Coordinates: 4152381..4153181 (+)
• Length: 266 a.a.
• NCBI ID: WP_021441214.1
• Uniprot ID: A0A4V0HF22
• Organism: Klebsiella pneumoniae strain SWHIN_100
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4147381..4158181

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUZ80_RS20300 (HUZ80_20265)	gspH	4147537..4148052 (+)	516	WP_023282194.1	type II secretion system minor pseudopilin GspH	-
HUZ80_RS20305 (HUZ80_20270)	gspI	4148049..4148414 (+)	366	WP_023282193.1	type II secretion system minor pseudopilin GspI	-
HUZ80_RS20310 (HUZ80_20275)	gspJ	4148411..4149007 (+)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
HUZ80_RS20315 (HUZ80_20280)	gspK	4149004..4149990 (+)	987	WP_023282192.1	type II secretion system minor pseudopilin GspK	-
HUZ80_RS20320 (HUZ80_20285)	gspL	4149977..4151170 (+)	1194	WP_032418425.1	type II secretion system protein GspL	-
HUZ80_RS20325 (HUZ80_20290)	-	4151163..4151648 (+)	486	WP_023282190.1	type II secretion system protein M	-
HUZ80_RS20330 (HUZ80_20295)	-	4151638..4152384 (+)	747	WP_023282189.1	type II secretion system protein N	-
HUZ80_RS20335 (HUZ80_20300)	pilD	4152381..4153181 (+)	801	WP_021441214.1	A24 family peptidase	Machinery gene
HUZ80_RS20340 (HUZ80_20305)	hrpB	4153229..4155658 (-)	2430	WP_023282188.1	ATP-dependent helicase HrpB	-
HUZ80_RS20345 (HUZ80_20310)	thpR	4155731..4156267 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
HUZ80_RS20350 (HUZ80_20315)	sfsA	4156267..4156983 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HUZ80_RS20355 (HUZ80_20320)	dksA	4157146..4157601 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=456355 HUZ80_RS20335 WP_021441214.1 4152381..4153181(+) (pilD) [Klebsiella pneumoniae strain SWHIN_100]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
SRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=456355 HUZ80_RS20335 WP_021441214.1 4152381..4153181(+) (pilD) [Klebsiella pneumoniae strain SWHIN_100]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
AGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
TCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

3D structure

• AlphaFold DB: A0A4V0HF22

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425