Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HUK74_RS25785 Genome accession   NZ_CP054791
Coordinates   5455363..5456235 (+) Length   290 a.a.
NCBI ID   WP_003094651.1    Uniprot ID   A0AAQ3LID6
Organism   Pseudomonas aeruginosa strain SE5430     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5450363..5461235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK74_RS25760 (HUK74_25725) nadC 5450690..5451537 (+) 848 Protein_5114 carboxylating nicotinate-nucleotide diphosphorylase -
  HUK74_RS25770 (HUK74_25735) - 5451741..5452205 (-) 465 WP_031630343.1 pilin -
  HUK74_RS25775 (HUK74_25740) pilB 5452435..5454135 (+) 1701 WP_003094647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUK74_RS25780 (HUK74_25745) pilC 5454139..5455359 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  HUK74_RS25785 (HUK74_25750) pilD 5455363..5456235 (+) 873 WP_003094651.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HUK74_RS25790 (HUK74_25755) coaE 5456232..5456843 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  HUK74_RS25795 (HUK74_25760) yacG 5456840..5457040 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HUK74_RS25800 (HUK74_25765) - 5457077..5457286 (-) 210 WP_003094660.1 hypothetical protein -
  HUK74_RS25805 (HUK74_25770) - 5457392..5458081 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  HUK74_RS25810 (HUK74_25775) - 5458078..5458548 (-) 471 WP_003094664.1 hypothetical protein -
  HUK74_RS25815 (HUK74_25780) - 5458545..5458970 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -
  HUK74_RS25820 (HUK74_25785) - 5459103..5459732 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  HUK74_RS25825 (HUK74_25790) - 5459729..5460178 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  HUK74_RS25830 (HUK74_25795) - 5460204..5460374 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31852.89 Da        Isoelectric Point: 7.7068

>NTDB_id=454638 HUK74_RS25785 WP_003094651.1 5455363..5456235(+) (pilD) [Pseudomonas aeruginosa strain SE5430]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQANYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=454638 HUK74_RS25785 WP_003094651.1 5455363..5456235(+) (pilD) [Pseudomonas aeruginosa strain SE5430]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGAACTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGTCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.516

96.897

0.538
  pilD Vibrio campbellii strain DS40M4

52.65

97.586

0.514
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

48.921

95.862

0.469