Detailed information NTG0454396

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HUS00_RS22280
• Genome accession: NZ_CP054768
• Coordinates: 4485305..4486105 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain KP20194b
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4480305..4491105

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUS00_RS22245 (HUS00_22245)	gspH	4480461..4480976 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
HUS00_RS22250 (HUS00_22250)	gspI	4480973..4481338 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HUS00_RS22255 (HUS00_22255)	gspJ	4481335..4481931 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
HUS00_RS22260 (HUS00_22260)	gspK	4481928..4482914 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
HUS00_RS22265 (HUS00_22265)	gspL	4482901..4484094 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
HUS00_RS22270 (HUS00_22270)	-	4484087..4484572 (+)	486	WP_004151943.1	type II secretion system protein M	-
HUS00_RS22275 (HUS00_22275)	-	4484562..4485308 (+)	747	WP_004145897.1	type II secretion system protein N	-
HUS00_RS22280 (HUS00_22280)	pilD	4485305..4486105 (+)	801	WP_002888947.1	A24 family peptidase	Machinery gene
HUS00_RS22285 (HUS00_22285)	hrpB	4486153..4488582 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
HUS00_RS22290 (HUS00_22290)	thpR	4488655..4489191 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
HUS00_RS22295 (HUS00_22295)	sfsA	4489191..4489907 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HUS00_RS22300 (HUS00_22300)	dksA	4490070..4490525 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=454396 HUS00_RS22280 WP_002888947.1 4485305..4486105(+) (pilD) [Klebsiella pneumoniae strain KP20194b]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=454396 HUS00_RS22280 WP_002888947.1 4485305..4486105(+) (pilD) [Klebsiella pneumoniae strain KP20194b]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425