Detailed information NTG0453308

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HUF98_RS14420
• Genome accession: NZ_CP054560
• Coordinates: 2992551..2993669 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain YC103
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2987551..2998669

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUF98_RS14410 (HUF98_14340)	-	2990667..2991359 (-)	693	WP_140952350.1	YggS family pyridoxal phosphate-dependent enzyme	-
HUF98_RS14415 (HUF98_14345)	pilT	2991486..2992523 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HUF98_RS14420 (HUF98_14350)	pilU	2992551..2993669 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HUF98_RS14425 (HUF98_14355)	fur	2993739..2994176 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HUF98_RS14430 (HUF98_14360)	-	2994288..2994686 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HUF98_RS14435 (HUF98_14365)	-	2994741..2995064 (-)	324	WP_005139957.1	RnfH family protein	-
HUF98_RS14440 (HUF98_14370)	-	2995064..2996131 (-)	1068	WP_002084046.1	hypothetical protein	-
HUF98_RS14445 (HUF98_14375)	-	2996284..2996739 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HUF98_RS14450 (HUF98_14380)	-	2996992..2997840 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=453308 HUF98_RS14420 WP_000347034.1 2992551..2993669(+) (pilU) [Acinetobacter baumannii strain YC103]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=453308 HUF98_RS14420 WP_000347034.1 2992551..2993669(+) (pilU) [Acinetobacter baumannii strain YC103]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39