Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HUE42_RS08865 Genome accession   NZ_CP054505
Coordinates   2034840..2036201 (-) Length   453 a.a.
NCBI ID   WP_014166726.1    Uniprot ID   G8XBQ8
Organism   Flavobacterium columnare strain F2R60     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2029840..2041201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUE42_RS08850 (HUE42_08860) - 2030649..2032271 (-) 1623 WP_014166723.1 DUF4139 domain-containing protein -
  HUE42_RS08855 (HUE42_08865) - 2032337..2034304 (-) 1968 WP_065213805.1 transglycosylase domain-containing protein -
  HUE42_RS08860 (HUE42_08870) - 2034313..2034828 (-) 516 WP_065213804.1 hypothetical protein -
  HUE42_RS08865 (HUE42_08875) radA 2034840..2036201 (-) 1362 WP_014166726.1 DNA repair protein RadA Machinery gene
  HUE42_RS08870 (HUE42_08880) - 2036208..2037473 (-) 1266 WP_014166727.1 alpha/beta hydrolase -
  HUE42_RS08875 (HUE42_08885) - 2037613..2038602 (-) 990 WP_014166728.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  HUE42_RS08880 (HUE42_08890) panD 2038577..2038927 (-) 351 WP_014166729.1 aspartate 1-decarboxylase -
  HUE42_RS08885 (HUE42_08895) panC 2038942..2039790 (-) 849 WP_077225477.1 pantoate--beta-alanine ligase -
  HUE42_RS08890 (HUE42_08900) - 2039898..2040707 (+) 810 WP_014166731.1 glycogen/starch synthase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50058.61 Da        Isoelectric Point: 6.1953

>NTDB_id=453036 HUE42_RS08865 WP_014166726.1 2034840..2036201(-) (radA) [Flavobacterium columnare strain F2R60]
MAKIKTSFFCQSCGSQFAKWQGQCTSCKEWNTLVEEIVQKEEKVTWKTESPTHKRAPKPLLVHEIDSTEEIRMNTTDGEL
NRVLGGGLVPGSLTLLGGEPGIGKSTLLLQISLKLPYKTLYVSGEESQKQIKMRAERITTKNDNCYILTETKTQNIFRQI
ETIEPDVVIIDSIQTLQTDYIESSAGSISQIKECTAELIKFAKETNTPVILIGHITKDGTIAGPKILEHMVDTVLQFEGD
RNHIYRILRSLKNRFGSTSELGIYEMLGSGLREVSNPSEILISHKDEELSGTAIATTMEGMRPLMIEIQALVSTAVYGTP
QRSTTGYNAKRLNMILAVLEKRAGFRLGSKDVFLNITGGITVDDPAIDLAVVAAILSSNEDIPVGKDMCFAGEVGLSGEI
RPVNRIDQRIQEAEKLGFSTIFVSKYSKISLKNHQITVKLVAKIEDVISELFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=453036 HUE42_RS08865 WP_014166726.1 2034840..2036201(-) (radA) [Flavobacterium columnare strain F2R60]
ATGGCCAAAATAAAAACCTCTTTTTTTTGTCAAAGCTGCGGCAGTCAGTTTGCTAAATGGCAAGGACAGTGTACCTCTTG
TAAAGAATGGAATACTCTAGTTGAAGAAATAGTTCAAAAAGAAGAAAAAGTGACTTGGAAAACCGAGTCTCCTACTCATA
AAAGAGCTCCAAAGCCCCTTTTAGTTCATGAAATTGATTCTACGGAAGAAATCCGAATGAACACAACTGATGGAGAATTA
AACCGCGTTTTAGGTGGCGGATTAGTTCCAGGTTCACTAACCTTATTAGGAGGTGAACCTGGCATTGGAAAGAGTACTTT
ATTGCTTCAAATATCTCTTAAGTTACCTTATAAAACGCTATATGTTTCAGGTGAAGAAAGCCAGAAACAAATAAAAATGC
GAGCAGAACGAATTACTACCAAAAATGATAACTGTTATATATTAACAGAAACCAAAACTCAAAACATTTTTAGACAAATT
GAAACGATTGAACCCGATGTGGTTATAATAGATTCCATACAAACTTTACAAACGGATTATATAGAATCCTCAGCAGGCAG
TATTTCTCAAATTAAAGAATGTACGGCAGAGCTTATTAAATTTGCTAAAGAAACCAATACCCCTGTCATTCTAATTGGTC
ATATAACAAAGGATGGCACCATTGCAGGACCAAAGATCTTAGAACACATGGTAGATACCGTATTGCAGTTTGAAGGAGAT
CGAAATCATATTTATCGCATTTTACGATCGCTCAAAAACCGTTTTGGTTCTACATCCGAATTGGGTATTTATGAAATGCT
TGGATCAGGACTTAGAGAGGTTAGTAATCCTTCTGAAATACTAATATCTCATAAGGATGAAGAATTATCAGGCACCGCTA
TAGCCACAACCATGGAAGGTATGCGCCCTCTAATGATTGAAATACAAGCACTCGTTTCTACAGCCGTATATGGAACACCT
CAAAGAAGCACTACGGGTTATAATGCCAAAAGACTCAATATGATACTTGCTGTTTTAGAAAAAAGAGCAGGCTTCCGATT
AGGGTCTAAAGACGTTTTTTTAAATATTACAGGCGGGATTACAGTAGATGATCCAGCCATTGACCTAGCAGTTGTGGCCG
CCATACTTTCTTCTAACGAAGATATTCCCGTAGGAAAAGATATGTGTTTTGCAGGCGAAGTAGGATTATCAGGAGAAATA
CGCCCCGTTAATAGAATTGATCAACGAATACAAGAAGCGGAAAAACTTGGTTTTTCAACCATATTTGTATCTAAATATAG
CAAAATATCATTGAAAAATCATCAAATAACAGTAAAACTAGTTGCCAAAATTGAAGATGTAATCAGTGAATTATTTGGAT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-219)

Subunit ChlI of Mg-chelatase (ChlI)

(315-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G8XBQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae R6

48.802

100

0.494
  radA Streptococcus pneumoniae Rx1

48.802

100

0.494
  radA Streptococcus pneumoniae D39

48.802

100

0.494
  radA Streptococcus mitis NCTC 12261

48.802

100

0.494
  radA Streptococcus pneumoniae TIGR4

48.802

100

0.494
  radA Streptococcus mitis SK321

48.802

100

0.494
  radA Bacillus subtilis subsp. subtilis str. 168

50.235

94.04

0.472