Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   HT132_RS14830 Genome accession   NZ_CP054479
Coordinates   3037403..3039232 (-) Length   609 a.a.
NCBI ID   WP_003155028.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain R8-25     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 3032403..3044232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT132_RS14795 (HT132_14795) yjbM 3032970..3033605 (-) 636 WP_003155019.1 GTP diphosphokinase -
  HT132_RS14800 (HT132_14800) - 3033633..3033998 (-) 366 WP_012117290.1 hypothetical protein -
  HT132_RS14805 (HT132_14805) - 3034123..3034695 (+) 573 WP_029973141.1 CYTH domain-containing protein -
  HT132_RS14810 (HT132_14810) - 3034700..3035386 (+) 687 WP_100261698.1 lytic transglycosylase domain-containing protein -
  HT132_RS14815 (HT132_14815) - 3035615..3036013 (+) 399 WP_100261697.1 thiol management oxidoreductase -
  HT132_RS14820 (HT132_14820) spxH 3036010..3036912 (+) 903 WP_003155024.1 protease adaptor protein SpxH -
  HT132_RS14825 (HT132_14825) - 3037198..3037365 (+) 168 WP_003155026.1 hypothetical protein -
  HT132_RS14830 (HT132_14830) pepF 3037403..3039232 (-) 1830 WP_003155028.1 oligoendopeptidase F Regulator
  HT132_RS14835 (HT132_14835) - 3039460..3040620 (-) 1161 WP_110124644.1 competence protein CoiA family protein -
  HT132_RS14840 (HT132_14840) mecA 3040771..3041427 (-) 657 WP_003155032.1 adaptor protein MecA Regulator
  HT132_RS14850 (HT132_14850) - 3041703..3042359 (+) 657 WP_014417421.1 TerC family protein -
  HT132_RS14855 (HT132_14855) spx 3042416..3042811 (-) 396 WP_003155034.1 transcriptional regulator Spx -
  HT132_RS14860 (HT132_14860) - 3042990..3043568 (-) 579 WP_007409110.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69799.43 Da        Isoelectric Point: 5.2024

>NTDB_id=452794 HT132_RS14830 WP_003155028.1 3037403..3039232(-) (pepF) [Bacillus amyloliquefaciens strain R8-25]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=452794 HT132_RS14830 WP_003155028.1 3037403..3039232(-) (pepF) [Bacillus amyloliquefaciens strain R8-25]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATCCCGAATTTATCTTCTTTTAAAGGCA
CGCTTGCTCATTCGGCTGATAATTTATACGCGGCGCTCACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCGCATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCCCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTCATTC
TGGAAAAAGAAGAGCTTAAGCTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCGCCTTCCAATACATTCAGTGTTTTAAATAACGCCGATAT
TACGTTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTCCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCCGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTTTACGCCCGTGTGAAAAAATATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATCCCTGAAGAAGTTTACGATAACCTGATTAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCATATTTATGACCTGTACACACCGCTTGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGATAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCTAC
GACCAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTGACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAACTGAATGAAATGGAAGAACTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488