Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   HT132_RS08895 Genome accession   NZ_CP054479
Coordinates   1717466..1718266 (+) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus amyloliquefaciens strain R8-25     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 1712466..1723266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT132_RS08875 (HT132_08875) - 1712588..1713433 (+) 846 WP_021494303.1 TlyA family RNA methyltransferase -
  HT132_RS08880 (HT132_08880) argR 1713530..1713979 (+) 450 WP_003153173.1 transcriptional regulator ArgR -
  HT132_RS08885 (HT132_08885) recN 1714011..1715741 (+) 1731 WP_007408359.1 DNA repair protein RecN Machinery gene
  HT132_RS08890 (HT132_08890) spoIVB 1715905..1717185 (+) 1281 WP_038461527.1 SpoIVB peptidase -
  HT132_RS08895 (HT132_08895) spo0A 1717466..1718266 (+) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  HT132_RS08900 (HT132_08900) alr 1718414..1719595 (-) 1182 WP_021494302.1 alanine racemase -
  HT132_RS08905 (HT132_08905) - 1719770..1719982 (-) 213 WP_014418351.1 spore germination protein -
  HT132_RS08910 (HT132_08910) - 1720108..1720320 (-) 213 WP_029973891.1 spore germination protein -
  HT132_RS08915 (HT132_08915) - 1720447..1721565 (-) 1119 WP_174532747.1 NADH-dependent flavin oxidoreductase -
  HT132_RS08920 (HT132_08920) - 1721659..1721796 (-) 138 WP_014470646.1 hypothetical protein -
  HT132_RS08925 (HT132_08925) - 1722093..1722725 (-) 633 WP_014418348.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=452782 HT132_RS08895 WP_003153177.1 1717466..1718266(+) (spo0A) [Bacillus amyloliquefaciens strain R8-25]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=452782 HT132_RS08895 WP_003153177.1 1717466..1718266(+) (spo0A) [Bacillus amyloliquefaciens strain R8-25]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCTGATGTACTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAATTCAGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97