Detailed information    

insolico Bioinformatically predicted

Overview

Name   recN   Type   Machinery gene
Locus tag   HT132_RS08885 Genome accession   NZ_CP054479
Coordinates   1714011..1715741 (+) Length   576 a.a.
NCBI ID   WP_007408359.1    Uniprot ID   I2C7K7
Organism   Bacillus amyloliquefaciens strain R8-25     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1709011..1720741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT132_RS08860 (HT132_08860) - 1709412..1709666 (+) 255 WP_007408352.1 exodeoxyribonuclease VII small subunit -
  HT132_RS08865 (HT132_08865) - 1709656..1710546 (+) 891 WP_053284918.1 polyprenyl synthetase family protein -
  HT132_RS08870 (HT132_08870) dxs 1710690..1712591 (+) 1902 WP_021494304.1 1-deoxy-D-xylulose-5-phosphate synthase -
  HT132_RS08875 (HT132_08875) - 1712588..1713433 (+) 846 WP_021494303.1 TlyA family RNA methyltransferase -
  HT132_RS08880 (HT132_08880) argR 1713530..1713979 (+) 450 WP_003153173.1 transcriptional regulator ArgR -
  HT132_RS08885 (HT132_08885) recN 1714011..1715741 (+) 1731 WP_007408359.1 DNA repair protein RecN Machinery gene
  HT132_RS08890 (HT132_08890) spoIVB 1715905..1717185 (+) 1281 WP_038461527.1 SpoIVB peptidase -
  HT132_RS08895 (HT132_08895) spo0A 1717466..1718266 (+) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  HT132_RS08900 (HT132_08900) alr 1718414..1719595 (-) 1182 WP_021494302.1 alanine racemase -
  HT132_RS08905 (HT132_08905) - 1719770..1719982 (-) 213 WP_014418351.1 spore germination protein -
  HT132_RS08910 (HT132_08910) - 1720108..1720320 (-) 213 WP_029973891.1 spore germination protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 64079.91 Da        Isoelectric Point: 4.7628

>NTDB_id=452781 HT132_RS08885 WP_007408359.1 1714011..1715741(+) (recN) [Bacillus amyloliquefaciens strain R8-25]
MLAELSIKNFAIIEELTISFERGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEFVRYGETKAELEGLFLLDSGHPVFEVC
REQGIDASDDMIVMRRDINAGGKSVCRVNGKLVTIAALREIGRLLLDIHGQHDNQLLMEDDKHLELLDRFAGAEAESALQ
AYHEGYERYMKLLKKVKKLSESEQEMAHRLDLIQFQLEEIESAKLELNEDELLQEERKQISNFEKIYESLQNAYNALRSE
QGGLDWVGMASAQLEDISDINEPLQKLSESVSSSYYLLEDATFQMRNLLDDLEYDPERLNFIETRLNEIKQLKRKYGATV
EDILEYGSKIEEEIDQIENRDSHLEALKKELESVGKDVAVEAANLSKIRKAWAKKLAEAIHQELKSLYMGKSTFDTEFLV
KTDPSASEAPVVNGQPVQLTQKGIDLVKFLISTNTGEPLKPLSKVASGGELSRVMLAMKSIFSSQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHKVSTGSQVLCITHLPQVAAMADTHLLIAKEFKDGRTTTHVTPLSKQDKVAEIGRMIAGVEVTDLTKR
HAQELLKQAGQVKTTG

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=452781 HT132_RS08885 WP_007408359.1 1714011..1715741(+) (recN) [Bacillus amyloliquefaciens strain R8-25]
TTGTTAGCTGAACTATCAATAAAAAACTTTGCCATTATAGAGGAACTGACGATTTCGTTTGAGCGGGGGCTCACCGTCCT
GACGGGAGAAACAGGGGCCGGCAAGTCAATTATTATAGACGCCGTTTCCCTATTAGTCGGCGGCCGCGGCTCGTCTGAAT
TTGTCCGTTACGGTGAAACAAAGGCCGAGCTTGAAGGACTGTTTCTATTGGACAGCGGTCATCCCGTGTTTGAAGTATGC
CGTGAGCAGGGAATAGACGCATCTGACGACATGATTGTCATGAGAAGGGACATCAATGCCGGAGGAAAAAGCGTCTGCCG
TGTCAACGGAAAATTAGTGACGATTGCGGCTTTAAGGGAAATCGGCAGACTTTTATTAGATATTCACGGACAGCATGATA
ACCAGCTGTTAATGGAGGACGATAAACACTTAGAGCTGTTGGACAGGTTTGCGGGCGCTGAGGCCGAATCCGCGCTTCAG
GCTTACCATGAAGGCTATGAGCGTTATATGAAGCTGCTCAAAAAAGTGAAGAAGCTGTCAGAAAGCGAGCAGGAAATGGC
GCATCGGTTAGACCTGATTCAATTTCAGCTTGAAGAAATCGAGTCGGCGAAGCTTGAATTGAATGAAGACGAGCTTCTTC
AGGAAGAGCGGAAGCAGATATCCAACTTTGAAAAAATTTATGAATCATTGCAAAACGCCTATAATGCGCTCCGCAGCGAG
CAGGGGGGGCTTGACTGGGTCGGAATGGCCTCTGCCCAGCTTGAAGATATTTCGGATATCAATGAACCGCTGCAAAAGCT
GTCAGAAAGCGTGTCAAGTTCCTATTATCTGCTGGAGGATGCCACCTTTCAGATGCGCAATCTGTTAGATGATCTGGAAT
ATGACCCGGAACGGCTGAATTTTATTGAAACCCGTTTAAACGAGATTAAACAGCTGAAGCGCAAATACGGAGCGACGGTA
GAAGACATTTTGGAATACGGCTCCAAAATAGAAGAGGAAATCGATCAAATCGAGAACCGCGACAGCCACCTTGAAGCGCT
GAAGAAGGAACTGGAGTCGGTCGGCAAAGATGTGGCCGTAGAAGCCGCCAATTTATCAAAAATCAGAAAAGCTTGGGCTA
AGAAACTGGCGGAAGCGATTCATCAGGAATTAAAAAGTCTGTATATGGGCAAATCAACGTTTGATACGGAATTTCTCGTG
AAAACCGATCCGTCAGCAAGTGAAGCGCCGGTTGTTAACGGGCAGCCCGTGCAGCTTACCCAAAAGGGGATTGACCTTGT
GAAGTTTTTAATTTCAACAAACACCGGTGAGCCTTTAAAACCGCTGTCAAAGGTCGCTTCCGGAGGCGAGCTTTCACGGG
TGATGCTGGCGATGAAGAGTATTTTTTCTTCCCAGCAGGATGTCACCTCCATCATCTTTGACGAAGTGGACACCGGTGTA
AGCGGGCGGGTTGCCCAAGCCATCGCGGAAAAAATCCATAAAGTATCGACCGGTTCACAGGTGCTCTGCATTACACACCT
GCCGCAGGTTGCCGCCATGGCGGATACGCACCTTCTGATCGCAAAAGAATTCAAAGACGGCCGGACGACAACGCATGTCA
CGCCGCTTTCCAAACAGGACAAGGTAGCGGAAATCGGCCGCATGATTGCGGGCGTTGAAGTGACCGATCTGACAAAACGG
CATGCACAAGAGCTTTTAAAACAGGCCGGACAGGTAAAAACGACAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_23)

(4-230)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recN Bacillus subtilis subsp. subtilis str. 168

88.715

100

0.887