Detailed information NTG0451961

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MS14393_RS26710
• Genome accession: NZ_CP054303
• Coordinates: 5452732..5453532 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain MS14393
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5447732..5458532

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MS14393_RS26690 (MS14393_05168)	dksA	5448312..5448767 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
MS14393_RS26695 (MS14393_05169)	sfsA	5448930..5449646 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
MS14393_RS26700 (MS14393_05170)	thpR	5449646..5450182 (-)	537	WP_040152504.1	RNA 2',3'-cyclic phosphodiesterase	-
MS14393_RS26705 (MS14393_05171)	hrpB	5450255..5452684 (+)	2430	WP_004147115.1	ATP-dependent helicase HrpB	-
MS14393_RS26710 (MS14393_05172)	pilD	5452732..5453532 (-)	801	WP_004145898.1	prepilin peptidase	Machinery gene
MS14393_RS26715 (MS14393_05173)	-	5453529..5454275 (-)	747	WP_004145897.1	type II secretion system protein N	-
MS14393_RS26720 (MS14393_05174)	-	5454265..5454750 (-)	486	WP_004151943.1	type II secretion system protein M	-
MS14393_RS26725 (MS14393_05175)	gspL	5454743..5455936 (-)	1194	WP_173820036.1	type II secretion system protein GspL	-
MS14393_RS26730 (MS14393_05176)	gspK	5455923..5456909 (-)	987	WP_077254241.1	type II secretion system minor pseudopilin GspK	-
MS14393_RS26735 (MS14393_05177)	gspJ	5456906..5457502 (-)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
MS14393_RS26740 (MS14393_05178)	gspI	5457499..5457864 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
MS14393_RS26745 (MS14393_05179)	gspH	5457861..5458376 (-)	516	WP_032414529.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=451961 MS14393_RS26710 WP_004145898.1 5452732..5453532(-) (pilD) [Klebsiella pneumoniae strain MS14393]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=451961 MS14393_RS26710 WP_004145898.1 5452732..5453532(-) (pilD) [Klebsiella pneumoniae strain MS14393]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGAGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425