Detailed information NTG0451647

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HL854_RS22190
• Genome accession: NZ_CP054254
• Coordinates: 4565491..4566291 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola strain FH-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4560491..4571291

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HL854_RS22155 (HL854_22415)	gspH	4560644..4561159 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
HL854_RS22160 (HL854_22420)	gspI	4561156..4561521 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
HL854_RS22165 (HL854_22425)	gspJ	4561518..4562114 (+)	597	WP_023297079.1	type II secretion system minor pseudopilin GspJ	-
HL854_RS22170 (HL854_22430)	gspK	4562111..4563091 (+)	981	WP_023297078.1	type II secretion system minor pseudopilin GspK	-
HL854_RS22175 (HL854_22435)	gspL	4563084..4564280 (+)	1197	WP_173818364.1	type II secretion system protein GspL	-
HL854_RS22180 (HL854_22440)	-	4564273..4564758 (+)	486	WP_012968921.1	type II secretion system protein M	-
HL854_RS22185 (HL854_22445)	-	4564748..4565494 (+)	747	WP_012968922.1	type II secretion system protein N	-
HL854_RS22190 (HL854_22450)	pilD	4565491..4566291 (+)	801	WP_008807384.1	prepilin peptidase	Machinery gene
HL854_RS22195 (HL854_22455)	hrpB	4566338..4568767 (-)	2430	WP_173818366.1	ATP-dependent helicase HrpB	-
HL854_RS22200 (HL854_22460)	thpR	4568840..4569376 (+)	537	WP_173818368.1	RNA 2',3'-cyclic phosphodiesterase	-
HL854_RS22205 (HL854_22465)	sfsA	4569376..4570092 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
HL854_RS22210 (HL854_22470)	dksA	4570255..4570710 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=451647 HL854_RS22190 WP_008807384.1 4565491..4566291(+) (pilD) [Klebsiella variicola strain FH-1]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=451647 HL854_RS22190 WP_008807384.1 4565491..4566291(+) (pilD) [Klebsiella variicola strain FH-1]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCACTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425