Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   I5367_RS13015 Genome accession   NZ_CP065539
Coordinates   2681677..2684034 (-) Length   785 a.a.
NCBI ID   WP_012118134.1    Uniprot ID   A7Z7E7
Organism   Bacillus amyloliquefaciens strain DGL1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2676677..2689034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5367_RS12990 (I5367_12990) - 2677183..2677962 (-) 780 WP_012118131.1 enoyl-CoA hydratase -
  I5367_RS12995 (I5367_12995) - 2677976..2678560 (-) 585 WP_007408119.1 TetR/AcrR family transcriptional regulator -
  I5367_RS13000 (I5367_13000) - 2678649..2680337 (-) 1689 WP_012118132.1 AMP-binding protein -
  I5367_RS13005 (I5367_13005) - 2680443..2681117 (+) 675 WP_012118133.1 DUF2711 family protein -
  I5367_RS13010 (I5367_13010) - 2681256..2681660 (-) 405 WP_003152541.1 DUF350 domain-containing protein -
  I5367_RS13015 (I5367_13015) mutS/mutS2 2681677..2684034 (-) 2358 WP_012118134.1 endonuclease MutS2 Machinery gene
  I5367_RS13020 (I5367_13020) polX 2684055..2685767 (-) 1713 WP_012118135.1 DNA polymerase/3'-5' exonuclease PolX -
  I5367_RS13025 (I5367_13025) - 2685873..2686406 (-) 534 WP_007612958.1 CvpA family protein -
  I5367_RS13030 (I5367_13030) zapA 2686414..2686671 (-) 258 WP_007408114.1 cell division protein ZapA -
  I5367_RS13035 (I5367_13035) rnhC 2686805..2687746 (+) 942 WP_012118136.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87233.51 Da        Isoelectric Point: 6.3216

>NTDB_id=450032 I5367_RS13015 WP_012118134.1 2681677..2684034(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain DGL1]
MQQKVLSSLEFHKVKEQITAHAASSLGREKLLQLKPLTDLSDIQKQLDEVEEASAVMRLRGHAPFGGLTDIRSALRRAEI
GSVLTPAEFTELSGLLYAVKQMKHFISQMTEDGVGIPLIQAHAEELITLGDLEREINSCIDDHGEVLDHASPALRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRMLTEHTAEHTQEIAQDVEVLQTLDFIFAKARYAKAMKATKPLMNGDGFIRLKKARHPLLPQDQVVAN
DIELGGDYSTIVITGPNTGGKTVTLKTLGLLTIMAQAGLHIPADEGSEAAVFDNVFADIGDEQSIEQSLSTFSSHMVNIV
NILKDVSENSLVLFDELGAGTDPQEGAALAMSILDEVHRTNARVLATTHYPELKAYGYNRQGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISRRLGLPEHIIGQAKSEMTAEHNEVDLMIASLEKSKKRADEELSETESIRKEAEKLHKDLQQQIIE
LNAQKDKMMEEAEQKAAEKLEAAANEAEQIIRELRSIKQEHRSFKEHELIDAKKRLGDAMPAFEKSKQPERKTEKKRELK
PGDEVKVLTFGQKGALLEKTGEKEWNVQIGILKMKVKEKDLEFLKSAPEPKKEKAITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGHPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTIVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=450032 I5367_RS13015 WP_012118134.1 2681677..2684034(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain DGL1]
GTGCAGCAAAAAGTGTTATCATCACTTGAATTTCATAAAGTAAAAGAACAGATTACCGCACATGCCGCCTCATCGCTCGG
CCGGGAGAAGCTTCTGCAGCTGAAACCTTTGACGGATCTCTCAGACATCCAAAAACAGCTGGATGAAGTCGAAGAAGCAT
CCGCGGTCATGCGCTTGAGAGGCCATGCTCCGTTCGGCGGCCTGACCGACATAAGATCCGCTTTACGGCGGGCGGAAATC
GGCAGTGTGCTGACGCCGGCGGAATTTACCGAATTGTCCGGGCTGCTGTATGCCGTTAAACAAATGAAGCATTTTATCAG
CCAAATGACAGAAGACGGCGTCGGCATTCCGCTGATACAGGCCCATGCAGAAGAGCTGATTACATTAGGCGATTTGGAAA
GGGAGATTAACTCCTGCATTGATGACCATGGGGAAGTGCTTGATCATGCGTCTCCCGCTTTAAGGGGAATCCGCACGCAG
CTCAGGACGCTTGAATCAAGAGTCAGAGACCGGCTTGAATCAATGCTGCGTTCATCTTCCGCTTCTAAAATGCTGTCTGA
CACCATCGTTACGATTCGGAATGACCGCTTTGTCATCCCGGTCAAACAGGAATACAGATCAAGCTACGGCGGAATTGTCC
ACGATACATCTTCATCCGGTGCGACACTGTTTATTGAACCGCAGGCGATCGTAGATATGAACAACTCGCTTCAGCAGGCG
AAAGTAAAAGAAAAACAGGAAATCGAACGGATTCTCCGAATGCTGACGGAGCATACGGCTGAACACACACAAGAAATCGC
TCAAGATGTGGAAGTGCTGCAGACACTGGATTTCATTTTCGCCAAAGCGAGATACGCAAAAGCGATGAAGGCGACAAAAC
CACTCATGAACGGAGACGGTTTTATCCGGCTGAAAAAAGCGCGCCATCCGCTGCTGCCCCAAGATCAAGTCGTGGCCAAC
GATATTGAACTGGGCGGAGATTACTCAACGATCGTTATCACAGGACCGAATACGGGCGGTAAAACCGTTACGTTAAAAAC
ACTCGGGCTGCTGACGATAATGGCGCAGGCGGGATTGCACATACCGGCTGATGAGGGATCGGAAGCAGCCGTATTTGACA
ATGTGTTTGCCGATATCGGCGATGAACAGTCGATCGAACAAAGTCTGAGTACGTTCTCATCCCACATGGTCAATATCGTC
AATATTTTAAAAGACGTGTCTGAAAACAGTCTTGTGCTGTTTGATGAACTCGGTGCCGGAACAGATCCGCAGGAAGGAGC
GGCGCTTGCCATGAGCATCCTTGACGAAGTTCACCGGACAAACGCCAGAGTGCTTGCGACAACCCATTATCCGGAACTGA
AAGCATACGGATATAACAGACAAGGCGTCATGAATGCCAGCGTTGAATTTGACATTGAAACGCTTTCGCCTACCTATAAG
CTTCTGATCGGAGTGCCGGGCCGAAGCAACGCATTTGAAATCTCAAGACGCCTTGGGCTTCCGGAGCATATCATCGGCCA
GGCGAAGTCAGAAATGACCGCCGAACATAACGAAGTCGATCTGATGATCGCATCGCTTGAAAAAAGCAAAAAAAGAGCGG
ATGAAGAGCTTTCTGAAACCGAATCAATCAGAAAAGAAGCAGAAAAACTGCACAAAGATCTTCAGCAGCAAATCATTGAG
CTGAACGCCCAAAAAGATAAAATGATGGAAGAAGCCGAACAGAAAGCTGCGGAAAAACTGGAAGCCGCCGCAAATGAAGC
CGAACAGATCATCCGCGAGCTCAGGTCCATCAAGCAAGAACACAGATCCTTTAAGGAACACGAGCTGATTGACGCCAAAA
AACGTCTCGGAGACGCGATGCCGGCTTTTGAAAAATCAAAGCAGCCGGAAAGAAAAACAGAGAAAAAACGCGAGCTGAAG
CCGGGCGACGAAGTAAAGGTGCTCACATTCGGACAAAAAGGGGCGCTGCTTGAGAAAACCGGCGAGAAAGAATGGAACGT
CCAAATCGGCATCCTCAAAATGAAGGTGAAGGAAAAAGATCTTGAGTTTCTCAAGTCCGCTCCTGAACCGAAAAAAGAAA
AAGCAATCACCGCCGTAAAAGGGAAGGATTATCACGTATCCCTTGAACTTGACCTCAGGGGAGAGCGTTACGAAAACGCC
CTCAGCCGCGTCGAAAAATATTTAGATGACGCGGTGCTTGCCGGCCATCCGAGAGTGTCCATCATTCACGGAAAAGGCAC
GGGCGCCCTCCGAAAAGGGGTGCAGGATCTTCTGAAAAACCACCGCAGCGTGAAAAGCTCGCGGTTCGGGGAAGCGGGAG
AAGGAGGATCAGGCGTAACGATTGTCGAACTAAAATAA

Domains

Predicted by InterProScan.

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)

Smr domain (Smr)

(710-785)

MutS domain V (MutS_V)

(331-512)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z7E7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

87.516

100

0.875