Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE   Type   Machinery gene
Locus tag   ST4147_RS08655 Genome accession   NZ_CP065504
Coordinates   1658342..1658635 (-) Length   97 a.a.
NCBI ID   WP_011226625.1    Uniprot ID   Q5M2G2
Organism   Streptococcus thermophilus strain 4147     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1653342..1663635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ST4147_RS08620 (ST4147_08595) - 1653740..1654188 (-) 449 Protein_1640 CAAX protease -
  ST4147_RS08625 - 1654262..1654687 (-) 426 WP_041827107.1 CPBP family intramembrane glutamic endopeptidase -
  ST4147_RS08630 (ST4147_08605) - 1654930..1655127 (-) 198 WP_011681682.1 helix-turn-helix transcriptional regulator -
  ST4147_RS08635 (ST4147_08610) - 1655376..1656569 (-) 1194 WP_022096898.1 acetate kinase -
  ST4147_RS08640 (ST4147_08615) comGH 1656625..1657581 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  ST4147_RS08645 (ST4147_08620) comGG 1657626..1657943 (-) 318 WP_002952035.1 competence type IV pilus minor pilin ComGG Machinery gene
  ST4147_RS08650 (ST4147_08625) comGF 1657921..1658358 (-) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  ST4147_RS08655 (ST4147_08630) comGE 1658342..1658635 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE Machinery gene
  ST4147_RS08660 (ST4147_08635) comGD 1658607..1659035 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  ST4147_RS08665 (ST4147_08640) comGC 1658995..1659321 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  ST4147_RS08670 (ST4147_08645) comGB 1659318..1660418 (-) 1101 WP_148148900.1 competence type IV pilus assembly protein ComGB Machinery gene
  ST4147_RS08675 (ST4147_08650) comGA/cglA/cilD 1660300..1661241 (-) 942 WP_011226629.1 competence type IV pilus ATPase ComGA Machinery gene
  ST4147_RS08680 (ST4147_08655) - 1661322..1661684 (-) 363 WP_011226630.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10844.74 Da        Isoelectric Point: 10.4561

>NTDB_id=449716 ST4147_RS08655 WP_011226625.1 1658342..1658635(-) (comGE) [Streptococcus thermophilus strain 4147]
MVNIKRRSLRGYILIESLVAMAVLVLVSSLILEQINTNRRLMADNLHQQEVLSVATMAVQTKQDQLTLNGIAVTVKRSQQ
GITVYESGKEIIHVSKQ

Nucleotide


Download         Length: 294 bp        

>NTDB_id=449716 ST4147_RS08655 WP_011226625.1 1658342..1658635(-) (comGE) [Streptococcus thermophilus strain 4147]
GTGGTAAATATAAAAAGACGATCTCTTAGAGGTTATATTCTTATTGAAAGTCTGGTAGCTATGGCTGTTTTAGTACTTGT
CAGCAGTCTGATATTGGAACAAATTAATACCAACCGCAGACTAATGGCTGATAATCTCCATCAACAAGAGGTCTTGAGTG
TGGCAACAATGGCAGTTCAGACCAAGCAAGACCAGTTAACGCTGAATGGCATAGCGGTGACAGTTAAACGTAGCCAGCAA
GGTATCACGGTTTATGAATCGGGAAAGGAGATTATTCATGTATCTAAGCAGTAA

Domains

Predicted by InterProScan.

Type II secretory pathway pseudopilin (ComGE)

(13-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M2G2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE Streptococcus mutans UA140

43.299

100

0.433
  comGE Streptococcus mutans UA159

43.299

100

0.433
  comGE Lactococcus lactis subsp. cremoris KW2

36.082

100

0.361