Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   HPY10_RS01340 Genome accession   NZ_CP053822
Coordinates   283292..283876 (+) Length   194 a.a.
NCBI ID   WP_000902746.1    Uniprot ID   -
Organism   Vibrio cholerae strain E7G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 278292..288876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY10_RS01325 (HPY10_01325) oxyR 278698..279591 (+) 894 WP_046121319.1 DNA-binding transcriptional regulator OxyR -
  HPY10_RS01330 (HPY10_01330) - 279666..282143 (-) 2478 WP_000668739.1 penicillin-binding protein 1A -
  HPY10_RS01335 (HPY10_01335) pilM 282286..283302 (+) 1017 WP_071171299.1 type IV pilus assembly protein PilM Machinery gene
  HPY10_RS01340 (HPY10_01340) pilN 283292..283876 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  HPY10_RS01345 (HPY10_01345) pilO 283869..284459 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY10_RS01350 (HPY10_01350) pilP 284449..284967 (+) 519 WP_032472751.1 pilus assembly protein PilP Machinery gene
  HPY10_RS01355 (HPY10_01355) pilQ 284993..286708 (+) 1716 WP_175245885.1 type IV pilus secretin PilQ Machinery gene
  HPY10_RS01360 (HPY10_01360) aroK 286895..287419 (+) 525 WP_000818616.1 shikimate kinase AroK -
  HPY10_RS01365 (HPY10_01365) aroB 287447..288532 (+) 1086 WP_175245886.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22022.45 Da        Isoelectric Point: 9.7294

>NTDB_id=447874 HPY10_RS01340 WP_000902746.1 283292..283876(+) (pilN) [Vibrio cholerae strain E7G]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQEGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=447874 HPY10_RS01340 WP_000902746.1 283292..283876(+) (pilN) [Vibrio cholerae strain E7G]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCCGCAGGCGAATACTTGGGCGGACAAATGGCTTTGCAGCAAGAGC
GGATTGGTTATTTGCAACAACATATCTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAGCGCAATAAAACGACTGAATTTATGAACCAAATGCC
CAACCTTATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCCCGCTTGGCAACCATGCTCGATAATTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGAGGGAGGTGCACACAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

99.485

100

0.995
  pilN Vibrio campbellii strain DS40M4

60.452

91.237

0.552