Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   HPY16_RS01305 Genome accession   NZ_CP053802
Coordinates   279578..280162 (+) Length   194 a.a.
NCBI ID   WP_000902746.1    Uniprot ID   -
Organism   Vibrio cholerae strain L6G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 274578..285162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY16_RS01290 (HPY16_01290) oxyR 274984..275877 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  HPY16_RS01295 (HPY16_01295) - 275952..278429 (-) 2478 WP_057555154.1 penicillin-binding protein 1A -
  HPY16_RS01300 (HPY16_01300) pilM 278572..279588 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  HPY16_RS01305 (HPY16_01305) pilN 279578..280162 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  HPY16_RS01310 (HPY16_01310) pilO 280155..280745 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY16_RS01315 (HPY16_01315) pilP 280735..281253 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  HPY16_RS01320 (HPY16_01320) pilQ 281279..282994 (+) 1716 WP_175247929.1 type IV pilus secretin PilQ Machinery gene
  HPY16_RS01325 (HPY16_01325) aroK 283181..283705 (+) 525 WP_000818616.1 shikimate kinase AroK -
  HPY16_RS01330 (HPY16_01330) aroB 283733..284818 (+) 1086 WP_000439809.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22022.45 Da        Isoelectric Point: 9.7294

>NTDB_id=447497 HPY16_RS01305 WP_000902746.1 279578..280162(+) (pilN) [Vibrio cholerae strain L6G]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQEGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=447497 HPY16_RS01305 WP_000902746.1 279578..280162(+) (pilN) [Vibrio cholerae strain L6G]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCCGCAGGCGAATACTTGGGCGGACAAATGGCTTTGCAGCAAGAGC
GGATTGGTTATTTGCAACAACATATCTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAGCGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGAGGGAGGTGCACACAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

99.485

100

0.995
  pilN Vibrio campbellii strain DS40M4

60.452

91.237

0.552