Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexA   Type   Machinery gene
Locus tag   I3J23_RS18300 Genome accession   NZ_CP065159
Coordinates   3596050..3598635 (+) Length   861 a.a.
NCBI ID   WP_048367486.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain GXU-1     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3591050..3603635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3J23_RS18280 (I3J23_18280) - 3591848..3593026 (+) 1179 WP_088037143.1 glycine C-acetyltransferase -
  I3J23_RS18285 (I3J23_18285) miaB 3593169..3594698 (+) 1530 WP_048367261.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  I3J23_RS18290 (I3J23_18290) ymcA 3594701..3595132 (+) 432 WP_003154113.1 RicAFT regulatory complex protein RicA family protein Regulator
  I3J23_RS18295 (I3J23_18295) cotE 3595386..3595931 (+) 546 WP_003154111.1 outer spore coat protein CotE -
  I3J23_RS18300 (I3J23_18300) hexA 3596050..3598635 (+) 2586 WP_048367486.1 DNA mismatch repair protein MutS Machinery gene
  I3J23_RS18305 (I3J23_18305) hexB 3598651..3600528 (+) 1878 WP_003154106.1 DNA mismatch repair endonuclease MutL Machinery gene
  I3J23_RS18310 (I3J23_18310) - 3600928..3601137 (-) 210 WP_048367263.1 hypothetical protein -
  I3J23_RS18315 (I3J23_18315) - 3602206..3602883 (+) 678 WP_003154103.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 861 a.a.        Molecular weight: 97701.90 Da        Isoelectric Point: 5.2532

>NTDB_id=445870 I3J23_RS18300 WP_048367486.1 3596050..3598635(+) (hexA) [Bacillus amyloliquefaciens strain GXU-1]
MASYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSEFRNGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVGAKEIVVSSKFNEHTAAQLKERCGATISIEDGEITERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERAGLIDPCGDLLDLLEEALHE
NPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAGLREKVKQFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGDSREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPDDLISRAQEILKQLEQTGDKPELPAAPEKK
PAVKEEPAQLSFFADGEKEQKAPAVSNKEKQVLEAFKSINILDMTPLEAMNEMYELQKKLK

Nucleotide


Download         Length: 2586 bp        

>NTDB_id=445870 I3J23_RS18300 WP_048367486.1 3596050..3598635(+) (hexA) [Bacillus amyloliquefaciens strain GXU-1]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGATAAAGGCAGAGCACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCGCAAGAGCTTGAAATTACACTGACAAGCAGAGACG
GAGGTTCGGCTGATAAAATTCCGATGTGCGGCGTGCCGTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAGCAGACGGAAGATCCGAAGGCGGCTAAAGGCGTTGTAAAAAGAGAAGTCGTCCAGCT
GATCACCCCGGGAACCGTGATGGATGGAAAAGGCATCCATGAGTCGGAAAACAACTTTATCGCTTCCGTTTCCGAATTTC
GTAACGGATACGGTCTCGCTTTATCAGACTTAACGACCGGAGAAAACCTGGCCGTATTTATTGAGCGGATTGAAGATGTC
ATGTCAGAAATTTACTCAGTCGGCGCAAAGGAAATTGTCGTATCAAGCAAGTTTAACGAACATACGGCTGCTCAGCTGAA
AGAACGGTGCGGCGCAACGATTTCTATCGAAGACGGGGAAATAACAGAACGCATTGAAATCGCAAAGCACTTGCCGGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAGAAAACGCAAAAACGGAGCCTGGATCATCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGACCTGTATTCAAAACGGAATCTTGAGCTGACGGAAACGATCCG
TTCGAAAAGCAAAAAAGGGTCATTGCTTTGGCTTTTAGACGAGACGAAAACAGCAATGGGCGGCCGGCTTCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCATCCCAAATCGAAGAACGCCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAGCGTGAAGATCTGCGCGAGCGCCTGAAGGAAGTATACGATTTAGAACGTCTGGCGGGCCGGGTCGCATTCGGAAACGT
TAACGCAAGGGATTTAATCCAGCTGAAAGAATCTTTAAAACAAGTGCCGAGCATTAAAGAGCTTGTAGGATCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCGGCTTGATTGATCCGTGCGGAGATTTGCTTGACCTGTTAGAAGAAGCCCTTCATGAA
AACCCGCCGTTATCGTTAAAAGAAGGCAACTTAATTAAAGACGGCTATCATCAGAAGCTTGATGAGTACCGTGACGCCAG
CAAAAACGGTAAAGACTGGATTGCCAGACTGGAGCAGCAGGAACGCGCTTATACGGGCATCCGTTCATTAAAGGTCGGTT
TCAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTGCTTGAGGACGGGCGGTATGAGCGGAAG
CAGACCCTCACAAACGCTGAACGGTACATTACGCCGGAATTGAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATCTGCGAGCTGGAATATGAGCTTTTTGCCGGTCTGCGTGAAAAGGTGAAACAATTTATTCCGCGTCTGCAGCGTCTTG
CCAAACAAATGAGTGAGCTGGATGCGCTGCAATGCTTTGCTACGATCAGTGAAAACAGGCATTACACAAAGCCTGTCTTT
TCTGATAATGAAGTAAAAGTCATTGAAGGGAGACACCCCGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTGCCGAA
CAACTGCCTGATGGGCGATTCGCGGGAAATGCTCCTGATTACGGGTCCGAATATGTCAGGTAAAAGCACGTACATGAGAC
AGATCGCACTTCTATCGATCATGGCCCAAATCGGCTGTTTTGTGCCCGCAAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTCACGAGAATCGGGGCAGCGGATGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCAATTGTGAACGCCACAAAAGACAGCCTGATCCTGTTTGATGAAATCGGCCGCGGGACATCAACGTACGACGGAATGG
CGCTGGCACAGGCGATTATTGAATATGTTCATGATCATATCGGCGCTAAAACACTGTTCAGCACGCATTATCATGAGCTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGCGCAGAGGAATATAACGGAACGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCAGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCGGAGCTCCCGGACGATCTGA
TCAGCCGCGCGCAGGAGATTTTGAAACAGCTTGAGCAGACGGGAGATAAACCGGAGCTTCCGGCGGCACCTGAGAAAAAG
CCCGCCGTCAAAGAAGAACCGGCACAGCTGTCCTTTTTTGCTGACGGTGAAAAAGAACAGAAGGCACCGGCGGTTTCTAA
TAAAGAAAAACAAGTGCTTGAAGCTTTTAAGTCAATCAATATTCTCGATATGACGCCGCTTGAGGCGATGAATGAAATGT
ACGAGCTGCAAAAAAAATTAAAATAG

Domains

Predicted by InterProScan.

MutS domain I (MutS_I)

(5-116)

MutS domain II (MutS_II)

(125-239)

MutS domain III (MutS_III)

(258-547)

MutS family domain IV (MutS_IV)

(415-506)

MutS domain V (MutS_V)

(598-785)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexA Streptococcus pneumoniae R6

48.308

99.535

0.481
  mutS Pseudomonas stutzeri strain ATCC 17587

38.549

100

0.395