Detailed information NTG0444394

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C5707_RS20385
• Genome accession: NZ_CP053364
• Coordinates: 4141224..4142024 (-)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain BA2275
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4136224..4147024

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C5707_RS20365 (C5707_020820)	dksA	4136804..4137259 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
C5707_RS20370 (C5707_020825)	sfsA	4137422..4138138 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
C5707_RS20375 (C5707_020830)	thpR	4138138..4138674 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
C5707_RS20380 (C5707_020835)	hrpB	4138747..4141176 (+)	2430	WP_171910911.1	ATP-dependent helicase HrpB	-
C5707_RS20385 (C5707_020840)	pilD	4141224..4142024 (-)	801	WP_002888947.1	prepilin peptidase	Machinery gene
C5707_RS20390 (C5707_020845)	-	4142021..4142767 (-)	747	WP_004145897.1	type II secretion system protein N	-
C5707_RS20395 (C5707_020850)	-	4142757..4143242 (-)	486	WP_004151943.1	type II secretion system protein M	-
C5707_RS20400 (C5707_020855)	gspL	4143235..4144428 (-)	1194	WP_004151942.1	type II secretion system protein GspL	-
C5707_RS20405 (C5707_020860)	gspK	4144415..4145401 (-)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
C5707_RS20410 (C5707_020865)	gspJ	4145398..4145994 (-)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
C5707_RS20415 (C5707_020870)	gspI	4145991..4146356 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
C5707_RS20420 (C5707_020875)	gspH	4146353..4146868 (-)	516	WP_171910912.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=444394 C5707_RS20385 WP_002888947.1 4141224..4142024(-) (pilD) [Klebsiella pneumoniae strain BA2275]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=444394 C5707_RS20385 WP_002888947.1 4141224..4142024(-) (pilD) [Klebsiella pneumoniae strain BA2275]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425