Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PMCN06_RS06080 Genome accession   NC_017027
Coordinates   1309617..1311002 (-) Length   461 a.a.
NCBI ID   WP_014391364.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. multocida str. HN06     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1304617..1316002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMCN06_RS06050 (PMCN06_1214) - 1304744..1306117 (-) 1374 WP_005722571.1 alanine/glycine:cation symporter family protein -
  PMCN06_RS06055 (PMCN06_1215) - 1306566..1306835 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  PMCN06_RS06060 (PMCN06_1216) yacG 1306835..1307038 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  PMCN06_RS06065 (PMCN06_1217) coaE 1307049..1307669 (-) 621 WP_014391361.1 dephospho-CoA kinase -
  PMCN06_RS06070 (PMCN06_1218) - 1307684..1308406 (-) 723 WP_041423102.1 prepilin peptidase -
  PMCN06_RS06075 (PMCN06_1219) - 1308406..1309617 (-) 1212 WP_014391363.1 type II secretion system F family protein -
  PMCN06_RS06080 (PMCN06_1220) pilB 1309617..1311002 (-) 1386 WP_014391364.1 GspE/PulE family protein Machinery gene
  PMCN06_RS06085 (PMCN06_1221) pilA 1311002..1311436 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  PMCN06_RS06090 (PMCN06_1223) ampD 1311596..1312162 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PMCN06_RS06095 (PMCN06_1224) rppH 1312748..1313344 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  PMCN06_RS06100 (PMCN06_1225) - 1313347..1314141 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  PMCN06_RS06105 (PMCN06_1226) lgt 1314151..1314963 (+) 813 WP_014391365.1 prolipoprotein diacylglyceryl transferase -
  PMCN06_RS06110 (PMCN06_1227) - 1314960..1315811 (+) 852 WP_014391366.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52255.91 Da        Isoelectric Point: 6.5608

>NTDB_id=44270 PMCN06_RS06080 WP_014391364.1 1309617..1311002(-) (pilB) [Pasteurella multocida subsp. multocida str. HN06]
MFHENPLCTVSTMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPHFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFTELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=44270 PMCN06_RS06080 WP_014391364.1 1309617..1311002(-) (pilB) [Pasteurella multocida subsp. multocida str. HN06]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTACAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAATATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTTCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCACTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTACTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGAAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.77

98.265

0.666
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

58.59

98.482

0.577
  pilB Acinetobacter baumannii D1279779

39.757

100

0.425
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio campbellii strain DS40M4

39.04

100

0.406
  pilB Vibrio cholerae strain A1552

38.272

100

0.403
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395

Multiple sequence alignment