Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HKW97_RS03490 Genome accession   NZ_CP053063
Coordinates   720634..722763 (+) Length   709 a.a.
NCBI ID   WP_407362835.1    Uniprot ID   -
Organism   Pseudomonas luteola strain JZ42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 715634..727763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKW97_RS03470 (HKW97_03455) comM 717832..718896 (+) 1065 WP_407362833.1 pilus assembly protein PilM Machinery gene
  HKW97_RS03475 (HKW97_03460) - 718896..719483 (+) 588 WP_010797031.1 PilN domain-containing protein -
  HKW97_RS03480 (HKW97_03465) pilO 719480..720103 (+) 624 WP_010797032.1 type 4a pilus biogenesis protein PilO -
  HKW97_RS03485 (HKW97_03470) pilP 720100..720624 (+) 525 WP_407362834.1 type 4a pilus biogenesis lipoprotein PilP -
  HKW97_RS03490 (HKW97_03475) pilQ 720634..722763 (+) 2130 WP_407362835.1 type IV pilus secretin PilQ Machinery gene
  HKW97_RS03495 (HKW97_03480) aroK 722769..723287 (+) 519 WP_219650794.1 shikimate kinase AroK -
  HKW97_RS03500 (HKW97_03485) aroB 723337..724440 (+) 1104 WP_407362836.1 3-dehydroquinate synthase -
  HKW97_RS03505 (HKW97_03490) - 724451..726055 (+) 1605 WP_407362837.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 709 a.a.        Molecular weight: 76014.32 Da        Isoelectric Point: 7.9084

>NTDB_id=442623 HKW97_RS03490 WP_407362835.1 720634..722763(+) (pilQ) [Pseudomonas luteola strain JZ42]
MMLHKTILQRVSGKSALTLAILLSGICPSLLLAADLKAMNVASLPGDRVELKLSFDQPVTAPKGYTIEQPARIALDLPGV
NNKLGARNKELGSGNARSVTIVEAGDRTRLIVNLTALAPYSTRVQGNDLFVVIGGDQAARTTSASVPAPSLPRSGAAQAP
APATNPAGRVFSSVPRVAGKAIRNIDFKRGDDGEGNVIIDLSETGISPDIQEQGGKIRLNFGKTQLPDALRVRLDVKDFA
TPVQFVNASSQAGDTVITIEPTAVYDYLAYQTDNKLTISVKPLTAKEKEQRAAERFTYTGEKLSLNFQDIDVRSVLQLIA
DFTGLNLVASDTVQGNITLRLQNVPWDQALDLVLKTKGLDKRQIGNVLLIAPADEIAARERQELESQKQIQELAPLRREL
IQVNYAKAADMAKLFQSVTKADAGQSERGSITVDDRTNSIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVNYN
KQLGVNWSGSRSGNGNFTISGGASSSTTGTTSPFVDLGVGNATSGIGIGFLTGSTTLDLQLSAMEASGNGEIVSQPKVVT
SDKETAKILRGTEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVNKDAPDYQNRLGNGAPPISKNEVNAK
VLVADGETIVIGGVFSNTQSKSTSKVPLLGDVPYVGRLFRRDSVTDEKVELLVFLTPRIMNNQAIAVGR

Nucleotide


Download         Length: 2130 bp        

>NTDB_id=442623 HKW97_RS03490 WP_407362835.1 720634..722763(+) (pilQ) [Pseudomonas luteola strain JZ42]
ATGATGTTGCACAAAACTATTCTACAAAGGGTCTCAGGGAAGAGTGCCCTCACGCTGGCCATACTCTTGAGCGGAATCTG
TCCCTCTTTGCTGCTGGCGGCAGATCTCAAGGCCATGAACGTGGCCTCTTTGCCGGGCGACAGAGTCGAACTCAAACTAT
CGTTCGATCAGCCAGTGACAGCCCCCAAGGGCTATACCATTGAGCAACCTGCGCGCATCGCTTTGGATCTGCCTGGCGTT
AATAACAAGCTAGGCGCTCGCAACAAGGAGTTGGGATCGGGGAATGCCAGAAGCGTTACCATAGTCGAGGCGGGTGACCG
CACGCGCCTTATCGTGAACCTGACTGCATTGGCGCCTTACTCGACCCGCGTTCAGGGTAACGACCTGTTCGTTGTCATTG
GCGGCGACCAGGCTGCTCGTACGACTAGTGCTTCTGTCCCGGCACCATCCTTGCCGCGCTCCGGTGCTGCGCAAGCCCCA
GCGCCTGCAACCAACCCTGCCGGCCGTGTCTTTTCCAGCGTGCCGAGAGTGGCTGGGAAAGCCATCAGGAATATCGACTT
CAAGCGAGGTGATGATGGAGAAGGTAACGTTATCATTGACCTTTCAGAAACAGGTATCAGCCCTGACATACAGGAGCAGG
GTGGAAAGATTCGCCTTAACTTCGGCAAGACGCAGCTCCCTGATGCTCTACGTGTTCGGCTTGACGTCAAAGACTTTGCT
ACGCCAGTACAGTTTGTAAACGCCTCAAGTCAGGCTGGTGACACGGTCATTACAATTGAGCCTACAGCCGTATATGACTA
TCTGGCCTACCAGACAGATAACAAGCTGACCATTAGCGTCAAGCCACTGACTGCCAAGGAAAAGGAACAGCGTGCCGCTG
AGCGTTTCACCTATACCGGTGAAAAGCTATCCCTGAACTTCCAGGATATTGATGTTCGCTCGGTCCTTCAGTTGATTGCT
GACTTCACCGGTTTGAACTTGGTGGCCAGCGACACCGTACAGGGCAATATCACGCTGCGCCTGCAGAACGTGCCGTGGGA
TCAGGCACTTGATCTTGTTCTGAAAACCAAGGGACTCGATAAGCGGCAAATAGGCAACGTATTGTTGATTGCGCCAGCGG
ATGAAATTGCGGCGCGTGAACGTCAGGAGCTGGAATCACAGAAGCAGATCCAAGAACTGGCGCCGCTACGCAGAGAGCTG
ATCCAGGTTAATTACGCCAAGGCTGCCGATATGGCCAAGCTGTTCCAGTCGGTTACTAAGGCTGATGCCGGACAAAGCGA
GCGTGGCTCCATTACCGTTGACGATCGGACAAACAGCATCATTGCCTATCAAACCCAGGATCGCCTGGATGAGCTGAGAC
GGATTGTCTCCCAGCTCGACATCCCGGTTCGCCAGGTAATGATCGAGGCACGAATTGTTGAGGCAAATGTCAACTACAAC
AAGCAGTTAGGCGTTAATTGGTCTGGCTCTCGCTCAGGTAATGGCAACTTTACCATTAGTGGGGGAGCTAGCTCTTCTAC
CACTGGCACAACCTCACCTTTTGTAGATTTGGGTGTGGGGAATGCGACGTCCGGTATTGGAATTGGATTCCTGACTGGTA
GCACAACATTGGATCTTCAGCTGTCTGCCATGGAAGCTAGTGGCAACGGTGAGATAGTTTCTCAACCCAAGGTGGTCACT
TCAGATAAGGAAACCGCAAAAATCTTGCGTGGTACTGAAATTCCGTATCAGGAGGCCAGTTCGAGCGGTGCGACCAGTAC
ATCCTTCAAGGAGGCAGCGCTTTCCTTGGAGGTAACGCCTCAGATCACGCCTGATAATCGAATCATCATGGAGGTTAAGG
TAAACAAGGATGCTCCTGATTATCAAAATCGCCTAGGTAATGGCGCTCCACCTATTTCCAAGAACGAAGTAAATGCCAAA
GTCTTGGTAGCTGATGGTGAGACTATTGTAATTGGGGGTGTTTTCTCGAACACACAAAGCAAGTCAACAAGTAAAGTGCC
TCTGTTAGGTGATGTACCTTACGTTGGACGCCTGTTCCGACGTGACTCGGTGACTGATGAAAAAGTAGAGCTGCTTGTCT
TCCTCACTCCGCGTATCATGAACAACCAAGCTATTGCCGTTGGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(543-700)

AMIN domain (AMIN)

(48-132)

Bacterial type II/III secretion system short domain (Secretin_N)

(400-465)

Secretin and TonB N terminus short domain (STN)

(326-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.59

100

0.743
  comQ Acinetobacter baylyi ADP1

40.788

100

0.409
  pilQ Acinetobacter baumannii strain A118

39.945

100

0.406
  pilQ Acinetobacter baumannii D1279779

39.804

100

0.402
  pilQ Legionella pneumophila strain ERS1305867

38.686

96.615

0.374
  pilQ Legionella pneumophila strain Lp02

38.686

96.615

0.374