Detailed information NTG0442236

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HKX07_RS23180
• Genome accession: NZ_CP053028
• Coordinates: 5014124..5014996 (+)
• Length: 290 a.a.
• NCBI ID: WP_003112839.1
• Uniprot ID: P22610
• Organism: Pseudomonas aeruginosa PAO1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5012996..5021604	5014124..5014996	within	0

Gene organization within MGE regions

Location: 5012996..5021604

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HKX07_RS23180	pilD	5014124..5014996 (+)	873	WP_003112839.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HKX07_RS23185	coaE	5014993..5015604 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
HKX07_RS23190	yacG	5015601..5015801 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
HKX07_RS23195	-	5015838..5016047 (-)	210	WP_003094660.1	hypothetical protein	-
HKX07_RS23200	-	5016153..5016842 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
HKX07_RS23205	-	5016839..5017309 (-)	471	WP_003103866.1	hypothetical protein	-
HKX07_RS23210	-	5017306..5017731 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
HKX07_RS23215	-	5017864..5018493 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
HKX07_RS23220	-	5018490..5018939 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
HKX07_RS23225	-	5018965..5019135 (+)	171	WP_003094672.1	DUF3094 family protein	-
HKX07_RS23230	-	5019199..5020506 (+)	1308	WP_003112837.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31869.92 Da
• Isoelectric Point: 7.7068

>NTDB_id=442236 HKX07_RS23180 WP_003112839.1 5014124..5014996(+) (pilD) [Pseudomonas aeruginosa PAO1]
MPLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=442236 HKX07_RS23180 WP_003112839.1 5014124..5014996(+) (pilD) [Pseudomonas aeruginosa PAO1]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: P22610

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	50.19	90.69	0.455