Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HKK53_RS18280 Genome accession   NZ_CP052861
Coordinates   3977496..3979586 (-) Length   696 a.a.
NCBI ID   WP_169371989.1    Uniprot ID   -
Organism   Pseudomonas sp. ADAK7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3972496..3984586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKK53_RS18265 (HKK53_18265) - 3974127..3975710 (-) 1584 WP_169371986.1 AAA family ATPase -
  HKK53_RS18270 (HKK53_18270) aroB 3975721..3976821 (-) 1101 WP_169371987.1 3-dehydroquinate synthase -
  HKK53_RS18275 (HKK53_18275) aroK 3976973..3977491 (-) 519 WP_169371988.1 shikimate kinase AroK -
  HKK53_RS18280 (HKK53_18280) pilQ 3977496..3979586 (-) 2091 WP_169371989.1 type IV pilus secretin PilQ Machinery gene
  HKK53_RS18285 (HKK53_18285) - 3979600..3980127 (-) 528 WP_169371990.1 pilus assembly protein PilP -
  HKK53_RS18290 (HKK53_18290) pilO 3980124..3980747 (-) 624 WP_169371991.1 type 4a pilus biogenesis protein PilO -
  HKK53_RS18295 (HKK53_18295) - 3980744..3981310 (-) 567 WP_169371992.1 PilN domain-containing protein -
  HKK53_RS18300 (HKK53_18300) comM 3981310..3982374 (-) 1065 WP_169371993.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 75214.53 Da        Isoelectric Point: 5.5794

>NTDB_id=441880 HKK53_RS18280 WP_169371989.1 3977496..3979586(-) (pilQ) [Pseudomonas sp. ADAK7]
MNRIFSTFGISLWIALLSPMVLAANLNALDVAALPGDRVELKLTFDGPPPPPHGYTTEQPARIALDLPGVVSKLANRNRD
LGGGNARSATVVEAKDRTRLIINLTQLAPYSSRVEGNNLFVVVGQGAKGTAPKPVASAPRTATATPAPAKAYAPAGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIILGFARTQLPEPLRVRLDVKDFATPVQFVNASATGDRAVISVEPSG
AFEYSTYQTDNKLTVSIRAVTADDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFTSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NNGANASSTIGTPGSTSTNSPFVDLGTAANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGT
EIPYQMASSSGATSVEFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVNDGETIVIGG
VFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=441880 HKK53_RS18280 WP_169371989.1 3977496..3979586(-) (pilQ) [Pseudomonas sp. ADAK7]
ATGAACAGGATTTTCTCAACCTTCGGTATTTCGCTATGGATAGCGCTGCTGTCGCCGATGGTACTAGCGGCCAACCTCAA
TGCGCTGGATGTCGCAGCGCTGCCGGGTGACCGCGTCGAGTTGAAGTTGACCTTCGACGGGCCGCCACCGCCGCCCCACG
GCTACACCACGGAGCAACCGGCGCGCATTGCGCTGGATCTGCCGGGTGTGGTCAGCAAACTGGCCAACAGGAATCGTGAC
CTGGGTGGCGGCAACGCCCGCAGTGCCACGGTGGTGGAGGCCAAGGACCGGACGCGACTGATCATCAACCTGACCCAGCT
GGCGCCCTACAGCTCCAGGGTTGAAGGCAACAATCTGTTCGTAGTCGTTGGGCAGGGCGCTAAAGGCACGGCGCCCAAAC
CCGTCGCCAGTGCTCCGCGCACCGCCACGGCCACGCCAGCCCCGGCGAAAGCCTACGCGCCGGCGGGCAAGGCCATCCGG
GGCGTGGACTTTCAGCGCGGCACCCAGGGTGAAGGCAATGTGGTGATCGACTTGTCGGATCCGTCCATCGCGCCGGACAT
TCAGGAACGGGACGGCAAGATCATCCTCGGTTTCGCCAGGACCCAATTGCCCGAGCCGTTGCGCGTGCGTCTCGACGTCA
AGGATTTCGCTACCCCGGTGCAATTCGTCAACGCCAGTGCCACCGGCGACCGTGCCGTGATCAGTGTTGAACCCAGCGGT
GCCTTCGAATATTCGACCTACCAGACCGACAACAAGCTGACCGTTAGCATCCGTGCGGTCACCGCCGACGACTTGCAGAA
GCGCAACGCCGAACGCTTTGCCTACACCGGTGAAAAACTCTCGCTGAATTTCCAGGACATCGATGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAACGCAAGATCGGCAACGTGTTGCTGGT
CGCGCCGGCCGATGAAATTGCCGCGCGGGAGCGTCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAGCTGGCGCCGCTGC
GTCGGGAGCTGTTGCAGGTCAACTACGCCAAGGCTGCGGATATCGCCAAGCTGTTTACCTCGGTGACCAGCGCCGAGGCG
AAAATCGATGAGCGCGGTTCGATCACCGTCGATGAACGGACCAATAACATCATTGCGTACCAGACCCAGGATCGCCTCGA
CGAGCTTCGGCGCATCGTCGCGCAGCTGGATATCCCGGTGCGCCAGGTAATGATCGAAGCGCGGATCGTCGAAGCCAATG
TCGATTATGACAAAAGTCTCGGCGTGCGCTGGGGTGGGTCGATCCAGAACAAGGGCAACTGGAACACGTCGGGCGTCAGC
AATAATGGGGCTAATGCTTCATCGACCATCGGTACGCCTGGCAGCACCAGCACCAACTCACCGTTCGTCGACTTGGGTAC
TGCGGCCAATACCTCGGGGCTCGGTATCGCTTTCATCACCGACAACGTACTGCTCGACCTCGAGCTGACGGCCATGGAGA
AGACCGGTAACGGCGAAATCGTCTCGCAGCCCAAGGTGGTCACGTCCGACAAGGAAACCGCGAAAATCCTCAAGGGCACG
GAAATTCCCTATCAGATGGCCAGCTCTAGCGGTGCGACATCAGTCGAATTCAAGGAGGCCTCGCTGTCCCTCGAAGTGAC
ACCGCAGATAACCCCGGACAACCGAATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTATCTGAACAAGCTCA
ACGATGTCCCGCCGATCAAGAAAAACGAAGTCAACGCCAAGGTGCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGG
GTTTTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCG
GCGTGACGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTGTTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTG
TGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(531-687)

AMIN domain (AMIN)

(38-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.706

100

0.757
  comQ Acinetobacter baylyi ADP1

42.06

100

0.422
  pilQ Acinetobacter baumannii strain A118

37.852

100

0.405
  pilQ Acinetobacter baumannii D1279779

37.852

100

0.405
  pilQ Legionella pneumophila strain Lp02

37.24

96.839

0.361
  pilQ Legionella pneumophila strain ERS1305867

37.24

96.839

0.361