Detailed information NTG0441496

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HH306_RS25290
• Genome accession: NZ_CP052759
• Coordinates: 5374698..5375570 (+)
• Length: 290 a.a.
• NCBI ID: WP_016562340.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain LYT4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5369698..5380570

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HH306_RS25270	-	5370241..5370939 (-)	699	WP_023122772.1	hypothetical protein	-
HH306_RS25275	-	5371003..5371542 (-)	540	WP_003116248.1	pilin	-
HH306_RS25280	pilB	5371771..5373474 (+)	1704	WP_003116249.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HH306_RS25285	pilC	5373478..5374695 (+)	1218	WP_003116250.1	type II secretion system F family protein	Machinery gene
HH306_RS25290	pilD	5374698..5375570 (+)	873	WP_016562340.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HH306_RS25295	coaE	5375567..5376178 (+)	612	WP_033982615.1	dephospho-CoA kinase	-
HH306_RS25300	yacG	5376175..5376375 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
HH306_RS25305	-	5376412..5376621 (-)	210	WP_003120946.1	hypothetical protein	-
HH306_RS25310	-	5376727..5377416 (-)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
HH306_RS25315	-	5377413..5377883 (-)	471	WP_003094664.1	hypothetical protein	-
HH306_RS25320	-	5377880..5378305 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
HH306_RS25325	-	5378438..5379067 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
HH306_RS25330	-	5379064..5379513 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
HH306_RS25335	-	5379539..5379709 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31843.83 Da
• Isoelectric Point: 7.3454

>NTDB_id=441496 HH306_RS25290 WP_016562340.1 5374698..5375570(+) (pilD) [Pseudomonas aeruginosa strain LYT4]
MELLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLASYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=441496 HH306_RS25290 WP_016562340.1 5374698..5375570(+) (pilD) [Pseudomonas aeruginosa strain LYT4]
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCTATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGCCAGCTACCTGGCGCTGGGCGGCAAGTGTTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTACTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.017	99.655	0.548
pilD	Vibrio campbellii strain DS40M4	53.497	98.621	0.528
pilD	Acinetobacter nosocomialis M2	50.352	97.931	0.493
pilD	Acinetobacter baumannii D1279779	50.352	97.931	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469