Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   INT78_RS09000 Genome accession   NZ_CP063774
Coordinates   1891773..1892582 (-) Length   269 a.a.
NCBI ID   WP_000464637.1    Uniprot ID   -
Organism   Escherichia coli O25b:H4-ST131     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1886773..1897582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INT78_RS08985 (INT78_08990) gspD 1888230..1890290 (-) 2061 WP_000498847.1 type II secretion system secretin GspD -
  INT78_RS08990 (INT78_08995) gspC 1890320..1891279 (-) 960 WP_001305091.1 type II secretion system protein GspC -
  INT78_RS08995 (INT78_09000) gspS2 1891297..1891707 (-) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  INT78_RS09000 (INT78_09005) pilD 1891773..1892582 (-) 810 WP_000464637.1 prepilin peptidase PppA Machinery gene
  INT78_RS09005 (INT78_09010) sslE 1892712..1897277 (-) 4566 WP_113224142.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29465.05 Da        Isoelectric Point: 8.3801

>NTDB_id=440773 INT78_RS09000 WP_000464637.1 1891773..1892582(-) (pilD) [Escherichia coli O25b:H4-ST131]
MFFDVFQQYPAAIPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWSESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=440773 INT78_RS09000 WP_000464637.1 1891773..1892582(-) (pilD) [Escherichia coli O25b:H4-ST131]
ATGTTTTTTGATGTTTTTCAGCAATACCCCGCGGCGATACCCGTCCTGGCTACCGTCGGAGGATTGATTATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGCCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGCCCACATTGTCAGCAGACCATCCGGATACGTGACAATATTCCG
CTGCTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGTGATTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACAGCACTCGCTTTTTTGCTGGCGAGTCTGGTGTGGTCAGAAAGTGGATGGGGACTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCATCATCGACCTCGATAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACC
GGGCTGATTGCGGCATGGGCGCAACAGAGTCCGTTAACGCTACAAGACGCAGTTACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCCG
CTTTAGGTAGTTGGGTGGGGCCGTTATCACTGCCCAATGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.318

99.257

0.45
  pilD Vibrio campbellii strain DS40M4

42.435

100

0.428
  pilD Neisseria gonorrhoeae MS11

39.3

95.539

0.375
  pilD Acinetobacter nosocomialis M2

38.113

98.513

0.375
  pilD Acinetobacter baumannii D1279779

37.736

98.513

0.372