Detailed information NTG0440271

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HER17_RS06740
• Genome accession: NZ_CP051652
• Coordinates: 1499036..1499881 (+)
• Length: 281 a.a.
• NCBI ID: WP_010302946.1
• Uniprot ID: A0AAI9L1D5
• Organism: Pectobacterium carotovorum strain WPP14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1494036..1504881

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HER17_RS06710 (HER17_06730)	gspI	1494447..1494812 (+)	366	WP_010302972.1	type II secretion system minor pseudopilin GspI	-
HER17_RS06715 (HER17_06735)	gspJ	1494812..1495462 (+)	651	WP_119881691.1	type II secretion system minor pseudopilin GspJ	-
HER17_RS06720 (HER17_06740)	gspK	1495459..1496445 (+)	987	WP_010302966.1	type II secretion system minor pseudopilin GspK	-
HER17_RS06725 (HER17_06745)	gspL	1496442..1497719 (+)	1278	WP_029368574.1	type II secretion system protein GspL	-
HER17_RS06730 (HER17_06750)	gspM	1497716..1498210 (+)	495	WP_010302957.1	type II secretion system protein GspM	-
HER17_RS06735 (HER17_06755)	-	1498200..1498946 (+)	747	WP_010302955.1	type II secretion system protein N	-
HER17_RS06740 (HER17_06760)	pilD	1499036..1499881 (+)	846	WP_010302946.1	A24 family peptidase	Machinery gene
HER17_RS06745 (HER17_06765)	ypdK	1500042..1500116 (-)	75	WP_099606229.1	membrane protein YpdK	-
HER17_RS06750 (HER17_06770)	-	1500473..1501498 (-)	1026	WP_010302944.1	LLM class flavin-dependent oxidoreductase	-
HER17_RS06755 (HER17_06775)	-	1501821..1502885 (+)	1065	WP_010302939.1	ABC transporter substrate-binding protein	-
HER17_RS06760 (HER17_06780)	-	1502882..1503919 (+)	1038	WP_010302936.1	iron ABC transporter permease	-
HER17_RS06765 (HER17_06785)	-	1503921..1504691 (+)	771	WP_010302930.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31271.14 Da
• Isoelectric Point: 6.4529

>NTDB_id=440271 HER17_RS06740 WP_010302946.1 1499036..1499881(+) (pilD) [Pectobacterium carotovorum strain WPP14]
MDDLREFAQLFPAWWFGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGVADSDPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLAAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSGTFVSLNDAVVGAMAGYLSLWLLYWAFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGLRKEDTAKPLAFGPWLAIGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=440271 HER17_RS06740 WP_010302946.1 1499036..1499881(+) (pilD) [Pectobacterium carotovorum strain WPP14]
GTGGACGATTTAAGGGAATTCGCACAGCTGTTTCCGGCGTGGTGGTTTGGGGCGCTGGGCGTGTTGGGGTTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCGTGGCCGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCTTGCCCGCACTGCCAGCAGGCTATT
GCGGTAAAAGATAATATCCCGCTATTCAGCTGGCTGTGGCTGCGCGGCCGCTCCCGCTGCTGCCATCAATCGGTCTCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGGCGGCAGGTTTGCTCTGGTTGCCCGGTATGGCGCTGT
GGGGGGCGTTGATCCTGCTGTCCTTCCTGTTGGTACTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATGGGGTTGCTGTTTAATCTGTCAGGGACATTTGTCTCATTGAATGATGCCGTAGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTACTTTATTGGGCATTCAAATACGCAACGGGCAAAGAAGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGGGCTTGGCTTGGCTGGCAGGCATTGCCCAATCTGGTTTTGGTGGCGGCGCTGAGT
GGGCTGGTCGTGACTCTCATCTGGCGCGGGTTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATCGGCGGGGTGTTCGGCGTGATAATGAACGGATTCAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.292	96.441	0.495
pilD	Vibrio campbellii strain DS40M4	47.037	96.085	0.452
pilD	Neisseria gonorrhoeae MS11	46.36	92.883	0.431
pilD	Acinetobacter baumannii D1279779	43.223	97.153	0.42
pilD	Acinetobacter nosocomialis M2	42.857	97.153	0.416