Detailed information NTG0439002

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HGK25_RS07765
• Genome accession: NZ_CP051490
• Coordinates: 1622494..1623294 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain M297-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1617494..1628294

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HGK25_RS07745 (HGK25_07925)	dksA	1618074..1618529 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
HGK25_RS07750 (HGK25_07930)	sfsA	1618692..1619408 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HGK25_RS07755 (HGK25_07935)	thpR	1619408..1619944 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
HGK25_RS07760 (HGK25_07940)	hrpB	1620017..1622446 (+)	2430	WP_004177388.1	ATP-dependent helicase HrpB	-
HGK25_RS07765 (HGK25_07945)	pilD	1622494..1623294 (-)	801	WP_004145898.1	A24 family peptidase	Machinery gene
HGK25_RS07770 (HGK25_07950)	-	1623291..1624037 (-)	747	WP_065888578.1	type II secretion system protein N	-
HGK25_RS07775 (HGK25_07955)	-	1624027..1624512 (-)	486	WP_004145896.1	type II secretion system protein M	-
HGK25_RS07780 (HGK25_07960)	gspL	1624505..1625698 (-)	1194	WP_048265188.1	type II secretion system protein GspL	-
HGK25_RS07785 (HGK25_07965)	gspK	1625685..1626671 (-)	987	WP_021467522.1	type II secretion system minor pseudopilin GspK	-
HGK25_RS07790 (HGK25_07970)	gspJ	1626668..1627264 (-)	597	WP_009309266.1	type II secretion system minor pseudopilin GspJ	-
HGK25_RS07795 (HGK25_07975)	gspI	1627261..1627626 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HGK25_RS07800 (HGK25_07980)	gspH	1627623..1628138 (-)	516	WP_004213437.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=439002 HGK25_RS07765 WP_004145898.1 1622494..1623294(-) (pilD) [Klebsiella pneumoniae strain M297-1]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=439002 HGK25_RS07765 WP_004145898.1 1622494..1623294(-) (pilD) [Klebsiella pneumoniae strain M297-1]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAACTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425