Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   HC660_RS05870 Genome accession   NZ_CP051464
Coordinates   1144126..1147830 (+) Length   1234 a.a.
NCBI ID   WP_168747367.1    Uniprot ID   -
Organism   Bacillus mojavensis strain UCMB5075     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1139126..1152830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC660_RS05855 (HC660_11160) - 1139573..1139887 (-) 315 WP_168747365.1 four-helix bundle copper-binding protein -
  HC660_RS05860 (HC660_11170) - 1140018..1140455 (-) 438 WP_024120837.1 ferritin family protein -
  HC660_RS05865 (HC660_11180) addB 1140639..1144139 (+) 3501 WP_168747366.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  HC660_RS05870 (HC660_11190) addA 1144126..1147830 (+) 3705 WP_168747367.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  HC660_RS05875 (HC660_11200) sbcD 1147902..1149080 (+) 1179 WP_168747368.1 exonuclease subunit SbcD -
  HC660_RS05880 (HC660_11210) sbcC 1149077..1152469 (+) 3393 WP_168747369.1 exonuclease subunit SbcC -
  HC660_RS05885 (HC660_11220) - 1152483..1152785 (+) 303 WP_168747370.1 HNH endonuclease -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141574.04 Da        Isoelectric Point: 5.1887

>NTDB_id=438466 HC660_RS05870 WP_168747367.1 1144126..1147830(+) (addA) [Bacillus mojavensis strain UCMB5075]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEEHPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVYEYSRSHPDPETWLKSFVHLYDVTEKSAIEELPFYPYVKEDIAMVLDGAKEKLQRA
LELTKAPGGPAPRAENFLDDLAQIDDLIQHQDDFNELYKRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGSGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPENNETETELLLIDKSEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFDAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLEPLAEGGAAAASDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAIRRGEPVPGPFAYEEKARERLAWKYSHHEVTQIRTKQSVSEIKRRREYEDEYSGRAPVTPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTTEEAERTVRHLYEKELLTEEQQSAIDIEEIVQFFHTEIGE
KLIGAKWKDREVPFSLALPAKEIYPDAKEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTRAVEQIAQTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=438466 HC660_RS05870 WP_168747367.1 1144126..1147830(+) (addA) [Bacillus mojavensis strain UCMB5075]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATCCT
TGTGGCAGCGGCGGCAGGATCCGGTAAAACCGCTGTTCTTGTGGAAAGAATGATCCGAAAAATCACGGCGGAAGAACATC
CGATCGATGTAGACCGGCTTCTTGTTGTCACCTTTACAAACGCCTCAGCTGCAGAGATGAAACACCGGATTGCGGATGCA
CTGGAAAAAGAGCTGGTAAAACGCCCAGGTTCGCTGCATATCCGTCGCCAGCTGTCTCTATTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTACCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAACTTCTCGGGGACGAGGTGCTTGATGAGCTGTTCGAGGATGAATATGCAAAGGGCGATAAGGCG
TTTTTTGAACTTGTCGACCGATACACAACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTATGAGTA
TTCCCGTTCCCACCCGGATCCGGAAACATGGCTGAAAAGCTTTGTTCATTTGTATGATGTAACAGAAAAGAGCGCGATCG
AGGAACTGCCGTTTTATCCATATGTCAAAGAAGATATTGCGATGGTGCTGGACGGAGCAAAGGAAAAGCTTCAGCGTGCT
CTTGAGCTGACAAAAGCGCCGGGCGGACCTGCGCCGCGCGCTGAAAATTTTCTCGATGATCTCGCGCAGATTGATGACCT
GATTCAGCATCAGGATGACTTCAATGAACTTTATAAGCGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAAG
GGGACGAGTTTGACCCCGTCTTGCTTGATGAAGCGACAGACTTGAGAAATGGCGCCAAAAAACTCCTTGAAAAACTCAAA
ACCGATTACTTCACCAGAAGCCCGGAGCAGCATTTAAAGAGTTTGGCTGACATGAAGCCTGTTATTGAAACACTCGTACA
GCTTGTCATCAGCTACGGGAAACGGTTTGAAGCCGCAAAGCAGGAGAAATCGCTCATTGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACCGCTGTGAACGAAAAGGGTGAACGTGAACCAAGTGAGGCGGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTCGATGAATATCAGGATACCAACCTGGTGCAGGAATCCATTCTTCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGAAACCTGTTTATGGTAGGAGATGTCAAGCAGTCGATTTATCGATTCAGGCTAGCGGAGCCGCTCCTTT
TTCTGTCTAAGTACAAACGGTTTACAGAGAGCGGAGAAGGGTCGGGCCGGAAAATCGACTTGAACAAAAACTTTCGAAGC
AGGGCTGATATTTTAGACAGCACAAACTTTTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTCGATTATGATGA
ACAGGCTGAACTGAAACTCGGCGCGGCGTATCCGGAAAACAATGAAACGGAAACCGAACTGCTGCTGATTGACAAGTCCG
AGGAGACGGACGCAGGAGAGGAAGCGGAAGAGCTGGAAACGGTACAGTTCGAGGCAAAAGCGATCGCCAAGGAAATACGC
AAGCTGATCTCATCGCCATTTAAGGTATATGACGGGAAAACGAAGACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTGCGATCCATGCCGTGGGCGCCGCAAATCATGGAAGAGCTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGCTACTTTGATGCGGTGGAAGTGTCTGTCGTGCTTTCTGTCCTGAAGGTGATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAACTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCGCCATACTATGAAGCAATGAAAGACTATCTGGCAGCCGGCGACCGAAACGATGAGCTTTATCAAAAGCTGCAAACGT
TTTACGGGCATTTGCAAAAATGGCGCGCATTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAGTATATGGATTATGTTGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCTAACTTGCGCGTCCTTTATGACCGGGC
ACGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGTTTCTTGAGGTTTATCGAACGCATGCAGGAACGCGGCGATG
ACCTGGGAACGGCGAGAGCGTTAAGCGAGCAGGAGGATGTTGTCCGCTTAATGACCATTCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTCTTCACAGCGGGACTTGGCCGGAATTTCAACATGATGGATCTCAACAAATCCTATCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCTGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCTGAGGAATTGCGTGTGCTCTATGTTGCTTTAACGCGGGCGAAGGAGAAGCTGTTTCTT
GTAGGCTCATGCAAGGATCATCAGAAACAGCTGGCAAAATGGCAGGCATCGGCATCCCAAGCTGACTGGCTCCTGCCGGA
ATTTGACCGCTATCAGGCGAAAACGTATTTGGATTTCATCGGACCTGCTCTTGTCAGACACAGAGACTTGGAGCCTTTGG
CTGAGGGCGGTGCGGCAGCGGCTTCTGATATATCAGGCCATCCGGCCCGCTTTGCTATTCAAATGATCCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAACGTCTTGAAGCGATCCGCCGCGGGGAACCGGTTCC
CGGCCCGTTTGCATATGAGGAAAAAGCTCGCGAGCGGCTGGCCTGGAAGTATTCGCATCACGAGGTGACACAAATTCGCA
CGAAGCAGTCTGTGTCTGAGATTAAGAGAAGAAGAGAGTACGAGGACGAATACAGCGGACGGGCACCCGTTACACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAGAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGACAGAAGAAGCGGAACGGACAGTTCGACATCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCGATATTGAGGAAATTGTTCAATTTTTCCATACCGAAATTGGCGAA
AAACTGATCGGCGCTAAATGGAAGGACCGTGAAGTTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GAAAGAGGCGGATGAGCCGCTATTGGTGCAGGGGATCATCGATTGTCTCTACGAAACAGAGGACGGCTTATATTTGCTGG
ACTATAAAACAGACCGGATTGAGGGGAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGAA
ACGCAAATTCAGCTGTACACAAGGGCCGTTGAACAAATTGCTCAAACGAAGGTAAAAGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACTTTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

93.598

100

0.936