Detailed information NTG0438465

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: HC660_RS05865
• Genome accession: NZ_CP051464
• Coordinates: 1140639..1144139 (+)
• Length: 1166 a.a.
• NCBI ID: WP_168747366.1
• Uniprot ID: -
• Organism: Bacillus mojavensis strain UCMB5075
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1135639..1149139

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HC660_RS05845 (HC660_11140)	-	1136490..1137692 (-)	1203	WP_168747363.1	MFS transporter	-
HC660_RS05850 (HC660_11150)	-	1137808..1139538 (+)	1731	WP_168747364.1	ABC transporter substrate-binding protein	-
HC660_RS05855 (HC660_11160)	-	1139573..1139887 (-)	315	WP_168747365.1	four-helix bundle copper-binding protein	-
HC660_RS05860 (HC660_11170)	-	1140018..1140455 (-)	438	WP_024120837.1	ferritin family protein	-
HC660_RS05865 (HC660_11180)	addB	1140639..1144139 (+)	3501	WP_168747366.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
HC660_RS05870 (HC660_11190)	addA	1144126..1147830 (+)	3705	WP_168747367.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
HC660_RS05875 (HC660_11200)	sbcD	1147902..1149080 (+)	1179	WP_168747368.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 134682.69 Da
• Isoelectric Point: 5.4309

>NTDB_id=438465 HC660_RS05865 WP_168747366.1 1140639..1144139(+) (addB) [Bacillus mojavensis strain UCMB5075]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDTGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMADSGTASEYRGERVLSE
KLHDLSILYQQMEKSLEGQYLHSEDYLTLLAEHIPQAEDLKGAHVYVDGFYQFTPQEFRVLEQLMLQAEHITFSLTADKP
SHEGEPQELDLFRMTGKTYYRLHQTAKELNLDITYKELDGLKRHKRVPELAHLEAQYEARPVIPFAEKQEAFTVMQAANR
RAELEGIAREIHSLVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKTSMMNHPLVEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNEPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDEFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGEDEI
SLDLFQQMMETGAESLTFSLIPPALDQVFVGNMDLSRIYGTSCTFVVGANDGVLPARPDENGVLSDDDREWLKTIGIELS
SGGRERLLDEHFLIYMALSSPSDRLYVTYPIADAEGKTLLPSIVIKRLEELFPQHRERLLTNEPEQVSDEEQLMYLVNKS
VAQSYTASQLRLWTREYEISDVWWSAYNALMNEPDRLESKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSLDWLGMKATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRQLKAEKDKTVLDWIKKEADGNEHS

Nucleotide

• Length: 3501 bp

>NTDB_id=438465 HC660_RS05865 WP_168747366.1 1140639..1144139(+) (addB) [Bacillus mojavensis strain UCMB5075]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAAGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATACGGGCGGCATGATTCGTGCTCAAGTCTTCAGCTTTTCACGACTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACGGGCGTACAGATGCTCCTGCGGAAGCTCATTGAAGAGCACAAACAGGAGTT
TAAAGTCTATCAAAAAGCAAGTGACAAAAGCGGTTTTACCGCACAGGTAGAACGGATGCTGACTGAGTTTAAGAGATACT
GTCTGGAACCGGAAGATATTCGCCGGATGGCGGATAGCGGAACAGCTTCCGAGTATCGAGGGGAACGTGTTTTATCTGAG
AAGCTTCATGATTTATCGATCCTGTACCAGCAGATGGAAAAAAGCCTTGAAGGTCAGTATCTTCATTCTGAGGATTATTT
GACACTATTGGCAGAGCATATCCCGCAGGCGGAAGATTTGAAAGGGGCACATGTCTATGTAGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTAGAGCAGCTTATGCTTCAGGCAGAGCATATCACGTTTTCACTCACAGCGGACAAGCCG
TCACATGAGGGGGAGCCGCAGGAACTTGATTTGTTCAGAATGACGGGAAAAACGTATTATCGCCTGCACCAGACGGCAAA
GGAACTGAATCTGGACATCACCTATAAAGAGCTTGATGGATTAAAACGGCACAAGCGCGTACCGGAATTAGCCCATTTGG
AAGCACAGTATGAAGCGCGTCCGGTTATTCCGTTTGCTGAGAAACAAGAGGCTTTTACCGTGATGCAGGCCGCAAACAGA
CGGGCAGAGCTGGAAGGTATTGCCCGGGAAATCCATAGTCTTGTCAGAGAGAAGGGCTACCGTTACAAGGATGTGGCGAT
TTTAGCGCGTCAGCCGGAAGACTACAAGGATATGGTCAAGGAAGTATTTGCAGATTACGAGATTCCTTATTTTATTGACG
GGAAAACGTCTATGATGAACCACCCGTTAGTGGAGTTTATTCGGTCAAGCCTTGACGTATTAAAGGGGAATTGGCGTTAT
GAAGCGGTGTTCCGCTGCGTGAAAACCGAACTGTTATTCCCGCTTAACGAGCCGAAGGCCAAGGTAAGAGAGCAGGTTGA
TCAGCTGGAGAATTACTGTATCGCTTACGGCATCAAAGGCGATCGCTGGACAAAGGGCGACCGTTTCCACTACAGACGTT
TTGTGTCGCTAGATGATGAATTTGCGCAGACCGATCAAGAAATTGAAATGGAAAACATGCTGAATGACACGCGCGATTGG
ATTGTTCCGCCGCTGTTCCAGCTGCAGAAACGAATGAAAAAAGCAAAAACGGTTCAAGAGAAGGCGGAGGCGCTTTACCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGGGCTGAGGAAGACGGCAGAATCATTGAAG
CACAGCAGCATCAGCAGGCATGGGACGCTGTCATTCAGCTGCTTGAGGAGTTTGTGGAAATGATGGGCGAAGATGAAATT
TCTCTTGATTTGTTTCAGCAGATGATGGAAACCGGTGCAGAGTCACTGACATTCTCTCTCATTCCTCCGGCGCTTGATCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATATACGGTACCTCATGTACCTTTGTGGTTGGGGCAAACGACGGCGTCC
TGCCGGCTCGCCCTGATGAAAATGGCGTGCTGTCGGATGATGATCGTGAGTGGCTGAAGACGATAGGTATTGAGCTTTCC
TCCGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTGATCTATATGGCGCTTTCTAGCCCATCTGACCGCCTTTATGT
GACGTATCCGATTGCTGATGCGGAAGGAAAAACGCTTTTGCCGTCGATTGTCATCAAGCGGCTGGAAGAACTGTTTCCGC
AGCATCGCGAGCGTCTATTGACAAATGAACCGGAGCAGGTCAGCGATGAGGAACAGCTAATGTACCTTGTCAATAAAAGC
GTGGCGCAATCATACACCGCTAGCCAGCTCAGATTATGGACGAGGGAATATGAGATCAGCGACGTCTGGTGGAGCGCGTA
CAATGCGCTGATGAATGAACCTGACAGGCTGGAGTCGAAAAAGCTTTTCTCAAGCCTGTTTTTCCGCAATGAAGTCAAGC
AGCTTGAACGTTCTGTATCGAGACAGCTCTATGGTGAACGTATTCAAGGGAGCGTGTCGAGGATGGAAACCTTTAACGCT
TGTCCCTTCTCCCATTTTGCGTCCCACGGGCTGCATCTGAAGGAACGTCAATTTTTCAAGCTTGAAGCACCGGATATCGG
GCAGCTCTTTCATTCCAGTTTAAAACTGATTTCAGACCGGCTTCGTGAGCAAAAATTAGATTGGCGCGACCTAACGAAGG
AGCAGTGCGAGCTGTTTTCTTATGACGCGGTCGAGCGGCTGGCGCCGAAGCTGCAAAAAGAAATTCTGCTCAGTTCAAAC
CGGCATTATTACGTGAAGGAAAAACTGCAGAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTGCCGATCGGCCTTGAATTGGGCTTTGGCGGAAAGGGCCCGCTCCCGCCGCTTACCTTTAAGCTGAAAAACG
GCTGTACGATGGAGCTCGTCGGGCGGATTGACCGCGTGGATAAAGCGGAAAGCTCTAAAGGCCTGCTCCTCCGGATTGTC
GATTATAAATCCAGTGATAAAGGCCTTGACTTGGCGGAAGTGTATTACGGACTTGCGCTGCAAATGCTGACGTATCTGGA
TTTATCGATTACACATTCATTAGACTGGCTAGGAATGAAAGCCACGCCGGCCGGGGTGCTGTATTTCCATATTCATGACC
CGATGATACAATCGAATCTTCCGCTTGGACTTGATGAGATTGAACAGGAAATTTTTAAGAAATTTAAGATGAAGGGCCTC
CTCCTCGGTGATCAGGAGGCGATTCGCCTCATGGATACAACCCTTCAAGAAGGAAGATCAAATATCATCAATGCGGGCTT
GAAGAAAGACGGCTCTCTCAGATCGGATTCAGCAGCAGTCGGTGAAAAAGAATTTGAGCTGTTGACGAAGCATGTGCGCC
GTACCTTCCAAGAAGCAGGCGAGCAAATCACTGACGGGCGCGTATCCATTGAGCCGTATAAAATGAAGAACAAGACACCT
TGCACATACTGTGCGTTTAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGTCAGTTAAAGGCCGA
AAAGGACAAGACGGTACTTGATTGGATAAAGAAGGAGGCTGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (306-669)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (790-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	94.768	100	0.948