Detailed information NTG0438169

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HGI13_RS08955
• Genome accession: NZ_CP051427
• Coordinates: 1907009..1907806 (+)
• Length: 265 a.a.
• NCBI ID: WP_182409635.1
• Uniprot ID: -
• Organism: Klebsiella michiganensis strain X2-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1902009..1912806

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HGI13_RS08920	gspH	1902150..1902662 (+)	513	WP_182409631.1	type II secretion system minor pseudopilin GspH	-
HGI13_RS08925	gspI	1902659..1903024 (+)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
HGI13_RS08930	gspJ	1903021..1903617 (+)	597	WP_182409632.1	type II secretion system minor pseudopilin GspJ	-
HGI13_RS08935	gspK	1903614..1904594 (+)	981	WP_109212803.1	type II secretion system minor pseudopilin GspK	-
HGI13_RS08940	gspL	1904587..1905780 (+)	1194	WP_182409633.1	type II secretion system protein GspL	-
HGI13_RS08945	-	1905773..1906258 (+)	486	WP_182409634.1	type II secretion system protein M	-
HGI13_RS08950	-	1906248..1907006 (+)	759	WP_004098694.1	type II secretion system protein N	-
HGI13_RS08955	pilD	1907009..1907806 (+)	798	WP_182409635.1	prepilin peptidase	Machinery gene
HGI13_RS08960	hrpB	1907904..1910333 (-)	2430	WP_049073913.1	ATP-dependent helicase HrpB	-
HGI13_RS08965	thpR	1910406..1910942 (+)	537	WP_182409636.1	RNA 2',3'-cyclic phosphodiesterase	-
HGI13_RS08970	sfsA	1910942..1911658 (+)	717	WP_004098690.1	DNA/RNA nuclease SfsA	-
HGI13_RS08975	dksA	1911823..1912278 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29082.73 Da
• Isoelectric Point: 7.5684

>NTDB_id=438169 HGI13_RS08955 WP_182409635.1 1907009..1907806(+) (pilD) [Klebsiella michiganensis strain X2-1]
METIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLFL
KGRSRCCGEPISPRYPLMELATGALFMLVGYLIPPGIPLLGGLIFVSVLLILAAIDAQTQLLPDRMTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=438169 HGI13_RS08955 WP_182409635.1 1907009..1907806(+) (pilD) [Klebsiella michiganensis strain X2-1]
ATGGAAACTATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGTTTCCTGTTTCTT
AAGGGACGTTCGCGCTGCTGCGGGGAACCCATCTCGCCTCGTTATCCTCTGATGGAGCTGGCGACCGGCGCGCTGTTTAT
GCTGGTCGGATACCTGATACCTCCGGGCATACCGCTGCTGGGCGGGCTAATTTTCGTATCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGATGACGCTGCCGCTGCTGTGGGCCGGGTTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCTGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.339	100	0.494
pilD	Neisseria gonorrhoeae MS11	47.826	95.472	0.457
pilD	Vibrio campbellii strain DS40M4	42.909	100	0.445
pilD	Acinetobacter nosocomialis M2	45.174	97.736	0.442
pilD	Acinetobacter baumannii D1279779	44.788	97.736	0.438