Detailed information NTG0437311

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HF896_RS05270
• Genome accession: NZ_CP051298
• Coordinates: 1062070..1062948 (-)
• Length: 292 a.a.
• NCBI ID: WP_013517807.1
• Uniprot ID: A0A858ZQU3
• Organism: Alicycliphilus denitrificans strain DP3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1057070..1067948

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HF896_RS05240 (HF896_05240)	argJ	1057748..1058977 (+)	1230	WP_013517801.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
HF896_RS05245 (HF896_05245)	-	1059032..1059904 (+)	873	WP_013517802.1	ATP-binding protein	-
HF896_RS05250 (HF896_05250)	-	1059901..1060326 (+)	426	WP_013517803.1	NUDIX domain-containing protein	-
HF896_RS05255 (HF896_05255)	-	1060389..1060595 (-)	207	WP_013517804.1	DNA gyrase inhibitor YacG	-
HF896_RS05260 (HF896_05260)	zapD	1060597..1061352 (-)	756	WP_013517805.1	cell division protein ZapD	-
HF896_RS05265 (HF896_05265)	coaE	1061435..1062049 (-)	615	WP_013517806.1	dephospho-CoA kinase	-
HF896_RS05270 (HF896_05270)	pilD	1062070..1062948 (-)	879	WP_013517807.1	A24 family peptidase	Machinery gene
HF896_RS05275 (HF896_05275)	pilC	1062948..1064165 (-)	1218	WP_013517808.1	type II secretion system F family protein	Machinery gene
HF896_RS05280 (HF896_05280)	pilB	1064187..1065926 (-)	1740	WP_013721478.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HF896_RS05285 (HF896_05285)	-	1066346..1067581 (+)	1236	WP_059400198.1	site-specific integrase	-

Sequence

Protein

• Length: 292 a.a.
• Molecular weight: 31415.02 Da
• Isoelectric Point: 7.8150

>NTDB_id=437311 HF896_RS05270 WP_013517807.1 1062070..1062948(-) (pilD) [Alicycliphilus denitrificans strain DP3]
MTGSAFADAVLVGVLGLLIGSFLNVVIHRLPRMMERQWAAECAQYAEDAGLPTQGGAAPTEQPFNLLTPRSRCPSCGHEV
RWYENIPVLSYLALRGRCSGCGTRIGVRYPLVELATAALFYACALRWGWSFTTLAWCGFCAALVALALIDWDTTLLPDDI
TLPLLWAGLLASVLRWIDVQPVDAVIGAAAGYVSLWLVYWGFKLATGKEGMGYGDFKLFAALGAWFGWQALVPIVLMASV
IGALVGIAMKFASSLREGKYVPFGPFLAGGGLAAMLWGPARIMRAIFSTLGL

Nucleotide

• Length: 879 bp

>NTDB_id=437311 HF896_RS05270 WP_013517807.1 1062070..1062948(-) (pilD) [Alicycliphilus denitrificans strain DP3]
ATGACGGGTTCCGCGTTTGCGGATGCCGTGCTCGTCGGCGTCCTGGGCCTGCTGATCGGCAGCTTTCTGAACGTGGTGAT
CCACCGCCTGCCGCGCATGATGGAACGGCAGTGGGCCGCGGAATGCGCGCAGTACGCCGAGGATGCGGGCTTGCCGACAC
AGGGCGGCGCCGCGCCGACCGAGCAGCCCTTCAACCTGCTGACGCCACGCTCGCGCTGTCCCTCCTGCGGGCACGAGGTG
CGCTGGTACGAGAACATTCCCGTGCTCAGCTATCTCGCACTGCGCGGCCGCTGCTCGGGCTGCGGCACGCGCATCGGCGT
GCGCTACCCGCTGGTCGAACTGGCCACCGCCGCCCTGTTCTATGCCTGCGCCCTGCGCTGGGGCTGGTCCTTCACGACGC
TGGCCTGGTGCGGCTTTTGCGCAGCCCTGGTGGCGCTGGCCCTCATCGACTGGGACACGACCCTGCTGCCCGACGACATC
ACCCTGCCCCTGCTGTGGGCCGGGCTGCTGGCCTCCGTGCTGCGCTGGATAGATGTGCAGCCGGTGGACGCGGTGATCGG
CGCGGCCGCGGGCTATGTATCGCTGTGGCTCGTCTACTGGGGCTTCAAGCTCGCCACGGGCAAGGAGGGCATGGGCTATG
GCGACTTCAAGCTGTTCGCGGCCCTGGGCGCGTGGTTCGGCTGGCAGGCCCTGGTGCCCATCGTCCTCATGGCGTCGGTC
ATCGGCGCCCTGGTAGGCATCGCCATGAAGTTCGCCAGCAGCCTGCGCGAGGGCAAGTACGTGCCCTTTGGCCCGTTCCT
GGCGGGAGGCGGGCTGGCCGCCATGCTGTGGGGGCCGGCGCGTATCATGCGTGCCATTTTCTCCACCCTGGGGCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (139-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-126)

3D structure

• AlphaFold DB: A0A858ZQU3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.175	97.603	0.49
pilD	Vibrio campbellii strain DS40M4	45.39	96.575	0.438
pilD	Neisseria gonorrhoeae MS11	47.71	89.726	0.428
pilD	Acinetobacter nosocomialis M2	43.223	93.493	0.404
pilD	Acinetobacter baumannii D1279779	43.223	93.493	0.404