Detailed information NTG0436353

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HF573_RS22075
• Genome accession: NZ_CP051160
• Coordinates: 4416246..4417046 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain KP20191015
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4411246..4422046

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HF573_RS22040 (HF573_22530)	gspH	4411402..4411917 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
HF573_RS22045 (HF573_22535)	gspI	4411914..4412279 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HF573_RS22050 (HF573_22540)	gspJ	4412276..4412872 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
HF573_RS22055 (HF573_22545)	gspK	4412869..4413855 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
HF573_RS22060 (HF573_22550)	gspL	4413842..4415035 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
HF573_RS22065 (HF573_22555)	-	4415028..4415513 (+)	486	WP_176608483.1	type II secretion system protein M	-
HF573_RS22070 (HF573_22560)	-	4415503..4416249 (+)	747	WP_004145897.1	type II secretion system protein N	-
HF573_RS22075 (HF573_22565)	pilD	4416246..4417046 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
HF573_RS22080 (HF573_22570)	hrpB	4417094..4419523 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
HF573_RS22085 (HF573_22575)	thpR	4419596..4420132 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
HF573_RS22090 (HF573_22580)	sfsA	4420132..4420848 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HF573_RS22095 (HF573_22585)	dksA	4421011..4421466 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=436353 HF573_RS22075 WP_002888947.1 4416246..4417046(+) (pilD) [Klebsiella pneumoniae strain KP20191015]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=436353 HF573_RS22075 WP_002888947.1 4416246..4417046(+) (pilD) [Klebsiella pneumoniae strain KP20191015]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425