Detailed information NTG0435204

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HC704_RS20730
• Genome accession: NZ_CP050845
• Coordinates: 4138530..4139330 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain Bckp206
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4133530..4144330

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HC704_RS20695 (HC704_20695)	gspH	4133686..4134201 (+)	516	WP_004183045.1	type II secretion system minor pseudopilin GspH	-
HC704_RS20700 (HC704_20700)	gspI	4134198..4134563 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HC704_RS20705 (HC704_20705)	gspJ	4134560..4135156 (+)	597	WP_004178612.1	type II secretion system minor pseudopilin GspJ	-
HC704_RS20710 (HC704_20710)	gspK	4135153..4136139 (+)	987	WP_004213439.1	type II secretion system minor pseudopilin GspK	-
HC704_RS20715 (HC704_20715)	gspL	4136126..4137319 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
HC704_RS20720 (HC704_20720)	-	4137312..4137797 (+)	486	WP_023284693.1	type II secretion system protein M	-
HC704_RS20725 (HC704_20725)	-	4137787..4138533 (+)	747	WP_031588870.1	type II secretion system protein N	-
HC704_RS20730 (HC704_20730)	pilD	4138530..4139330 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
HC704_RS20735 (HC704_20735)	hrpB	4139378..4141807 (-)	2430	WP_031588872.1	ATP-dependent helicase HrpB	-
HC704_RS20740 (HC704_20740)	thpR	4141880..4142416 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
HC704_RS20745 (HC704_20745)	sfsA	4142416..4143132 (+)	717	WP_004177390.1	DNA/RNA nuclease SfsA	-
HC704_RS20750 (HC704_20750)	dksA	4143295..4143750 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=435204 HC704_RS20730 WP_004145898.1 4138530..4139330(+) (pilD) [Klebsiella pneumoniae strain Bckp206]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=435204 HC704_RS20730 WP_004145898.1 4138530..4139330(+) (pilD) [Klebsiella pneumoniae strain Bckp206]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGACTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGTTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425