Detailed information NTG0435181

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HC697_RS21935
• Genome accession: NZ_CP050843
• Coordinates: 4357814..4358614 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain Bckp186
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4352814..4363614

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HC697_RS21900 (HC697_21900)	gspH	4352970..4353485 (+)	516	WP_167830300.1	type II secretion system minor pseudopilin GspH	-
HC697_RS21905 (HC697_21905)	gspI	4353482..4353847 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HC697_RS21910 (HC697_21910)	gspJ	4353844..4354440 (+)	597	WP_102014977.1	type II secretion system minor pseudopilin GspJ	-
HC697_RS21915 (HC697_21915)	gspK	4354437..4355423 (+)	987	WP_004183041.1	type II secretion system minor pseudopilin GspK	-
HC697_RS21920 (HC697_21920)	gspL	4355410..4356603 (+)	1194	WP_167830301.1	type II secretion system protein GspL	-
HC697_RS21925 (HC697_21925)	-	4356596..4357081 (+)	486	WP_004145896.1	type II secretion system protein M	-
HC697_RS21930 (HC697_21930)	-	4357071..4357817 (+)	747	WP_167830302.1	type II secretion system protein N	-
HC697_RS21935 (HC697_21935)	pilD	4357814..4358614 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
HC697_RS21940 (HC697_21940)	hrpB	4358662..4361091 (-)	2430	WP_167830303.1	ATP-dependent helicase HrpB	-
HC697_RS21945 (HC697_21945)	thpR	4361164..4361700 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
HC697_RS21950 (HC697_21950)	sfsA	4361700..4362416 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HC697_RS21955 (HC697_21955)	dksA	4362579..4363034 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=435181 HC697_RS21935 WP_002888947.1 4357814..4358614(+) (pilD) [Klebsiella pneumoniae strain Bckp186]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=435181 HC697_RS21935 WP_002888947.1 4357814..4358614(+) (pilD) [Klebsiella pneumoniae strain Bckp186]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAACTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425