Detailed information NTG0435157

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HC692_RS21650
• Genome accession: NZ_CP050840
• Coordinates: 4363022..4363822 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain Bckp101
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4358022..4368822

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HC692_RS21615 (HC692_21615)	gspH	4358178..4358693 (+)	516	WP_004213437.1	type II secretion system minor pseudopilin GspH	-
HC692_RS21620 (HC692_21620)	gspI	4358690..4359055 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HC692_RS21625 (HC692_21625)	gspJ	4359052..4359648 (+)	597	WP_004178612.1	type II secretion system minor pseudopilin GspJ	-
HC692_RS21630 (HC692_21630)	gspK	4359645..4360631 (+)	987	WP_004213439.1	type II secretion system minor pseudopilin GspK	-
HC692_RS21635 (HC692_21635)	gspL	4360618..4361811 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
HC692_RS21640 (HC692_21640)	-	4361804..4362289 (+)	486	WP_023284693.1	type II secretion system protein M	-
HC692_RS21645 (HC692_21645)	-	4362279..4363025 (+)	747	WP_031588870.1	type II secretion system protein N	-
HC692_RS21650 (HC692_21650)	pilD	4363022..4363822 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
HC692_RS21655 (HC692_21655)	hrpB	4363870..4366299 (-)	2430	WP_065808762.1	ATP-dependent helicase HrpB	-
HC692_RS21660 (HC692_21660)	thpR	4366372..4366908 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
HC692_RS21665 (HC692_21665)	sfsA	4366908..4367624 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HC692_RS21670 (HC692_21670)	dksA	4367787..4368242 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=435157 HC692_RS21650 WP_004145898.1 4363022..4363822(+) (pilD) [Klebsiella pneumoniae strain Bckp101]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=435157 HC692_RS21650 WP_004145898.1 4363022..4363822(+) (pilD) [Klebsiella pneumoniae strain Bckp101]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGACTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGTTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425