Detailed information NTG0435104

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HC709_RS21770
• Genome accession: NZ_CP050829
• Coordinates: 4343612..4344412 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain Bckp067
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4338612..4349412

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HC709_RS21735 (HC709_21735)	gspH	4338768..4339283 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
HC709_RS21740 (HC709_21740)	gspI	4339280..4339645 (+)	366	WP_065811636.1	type II secretion system minor pseudopilin GspI	-
HC709_RS21745 (HC709_21745)	gspJ	4339642..4340238 (+)	597	WP_023286045.1	type II secretion system minor pseudopilin GspJ	-
HC709_RS21750 (HC709_21750)	gspK	4340235..4341221 (+)	987	WP_021467522.1	type II secretion system minor pseudopilin GspK	-
HC709_RS21755 (HC709_21755)	gspL	4341208..4342401 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
HC709_RS21760 (HC709_21760)	-	4342394..4342879 (+)	486	WP_004145896.1	type II secretion system protein M	-
HC709_RS21765 (HC709_21765)	-	4342869..4343615 (+)	747	WP_004145897.1	type II secretion system protein N	-
HC709_RS21770 (HC709_21770)	pilD	4343612..4344412 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
HC709_RS21775 (HC709_21775)	hrpB	4344460..4346889 (-)	2430	WP_064145607.1	ATP-dependent helicase HrpB	-
HC709_RS21780 (HC709_21780)	thpR	4346962..4347498 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
HC709_RS21785 (HC709_21785)	sfsA	4347498..4348214 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HC709_RS21790 (HC709_21790)	dksA	4348377..4348832 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=435104 HC709_RS21770 WP_004145898.1 4343612..4344412(+) (pilD) [Klebsiella pneumoniae strain Bckp067]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=435104 HC709_RS21770 WP_004145898.1 4343612..4344412(+) (pilD) [Klebsiella pneumoniae strain Bckp067]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGTCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425