Detailed information NTG0435080

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HC649_RS21200
• Genome accession: NZ_CP050826
• Coordinates: 4231339..4232139 (+)
• Length: 266 a.a.
• NCBI ID: WP_032419388.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain Bckp212
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4226339..4237139

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HC649_RS21165 (HC649_21165)	gspH	4226495..4227010 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
HC649_RS21170 (HC649_21170)	gspI	4227007..4227372 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HC649_RS21175 (HC649_21175)	gspJ	4227369..4227965 (+)	597	WP_032419387.1	type II secretion system minor pseudopilin GspJ	-
HC649_RS21180 (HC649_21180)	gspK	4227962..4228948 (+)	987	WP_077259564.1	type II secretion system minor pseudopilin GspK	-
HC649_RS21185 (HC649_21185)	gspL	4228935..4230128 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
HC649_RS21190 (HC649_21190)	-	4230121..4230606 (+)	486	WP_004145896.1	type II secretion system protein M	-
HC649_RS21195 (HC649_21195)	-	4230596..4231342 (+)	747	WP_004145897.1	type II secretion system protein N	-
HC649_RS21200 (HC649_21200)	pilD	4231339..4232139 (+)	801	WP_032419388.1	prepilin peptidase	Machinery gene
HC649_RS21205 (HC649_21205)	hrpB	4232187..4234616 (-)	2430	WP_167813047.1	ATP-dependent helicase HrpB	-
HC649_RS21210 (HC649_21210)	thpR	4234689..4235225 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
HC649_RS21215 (HC649_21215)	sfsA	4235225..4235941 (+)	717	WP_004177390.1	DNA/RNA nuclease SfsA	-
HC649_RS21220 (HC649_21220)	dksA	4236104..4236559 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29477.07 Da
• Isoelectric Point: 8.6098

>NTDB_id=435080 HC649_RS21200 WP_032419388.1 4231339..4232139(+) (pilD) [Klebsiella pneumoniae strain Bckp212]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTEDDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLTPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=435080 HC649_RS21200 WP_032419388.1 4231339..4232139(+) (pilD) [Klebsiella pneumoniae strain Bckp212]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGAGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGACGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425