Detailed information NTG0435056

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HC645_RS20835
• Genome accession: NZ_CP050822
• Coordinates: 4184536..4185336 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain Bckp091
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4179536..4190336

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HC645_RS20800 (HC645_20800)	gspH	4179692..4180207 (+)	516	WP_021313445.1	type II secretion system minor pseudopilin GspH	-
HC645_RS20805 (HC645_20805)	gspI	4180204..4180569 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HC645_RS20810 (HC645_20810)	gspJ	4180566..4181162 (+)	597	WP_023342989.1	type II secretion system minor pseudopilin GspJ	-
HC645_RS20815 (HC645_20815)	gspK	4181159..4182145 (+)	987	WP_077254844.1	type II secretion system minor pseudopilin GspK	-
HC645_RS20820 (HC645_20820)	gspL	4182132..4183325 (+)	1194	WP_032428212.1	type II secretion system protein GspL	-
HC645_RS20825 (HC645_20825)	-	4183318..4183803 (+)	486	WP_004151943.1	type II secretion system protein M	-
HC645_RS20830 (HC645_20830)	-	4183793..4184539 (+)	747	WP_023316460.1	type II secretion system protein N	-
HC645_RS20835 (HC645_20835)	pilD	4184536..4185336 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
HC645_RS20840 (HC645_20840)	hrpB	4185384..4187813 (-)	2430	WP_023302300.1	ATP-dependent helicase HrpB	-
HC645_RS20845 (HC645_20845)	thpR	4187886..4188422 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
HC645_RS20850 (HC645_20850)	sfsA	4188422..4189138 (+)	717	WP_023342992.1	DNA/RNA nuclease SfsA	-
HC645_RS20855 (HC645_20855)	dksA	4189301..4189756 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=435056 HC645_RS20835 WP_004145898.1 4184536..4185336(+) (pilD) [Klebsiella pneumoniae strain Bckp091]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=435056 HC645_RS20835 WP_004145898.1 4184536..4185336(+) (pilD) [Klebsiella pneumoniae strain Bckp091]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425