Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   HCU65_RS05600 Genome accession   NZ_CP050705
Coordinates   1137379..1141083 (+) Length   1234 a.a.
NCBI ID   WP_219947334.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain PENSV20     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1132379..1146083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCU65_RS05585 (HCU65_05605) - 1132826..1133152 (-) 327 WP_219947774.1 four-helix bundle copper-binding protein -
  HCU65_RS05590 (HCU65_05610) - 1133249..1133686 (-) 438 WP_003327683.1 ferritin family protein -
  HCU65_RS05595 (HCU65_05615) addB 1133892..1137392 (+) 3501 WP_219947333.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  HCU65_RS05600 (HCU65_05620) addA 1137379..1141083 (+) 3705 WP_219947334.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  HCU65_RS05605 (HCU65_05625) sbcD 1141154..1142332 (+) 1179 WP_106034625.1 exonuclease subunit SbcD -
  HCU65_RS05610 (HCU65_05630) sbcC 1142329..1145727 (+) 3399 WP_219947335.1 exonuclease subunit SbcC -
  HCU65_RS05615 (HCU65_05635) - 1145744..1146046 (+) 303 WP_219947336.1 HNH endonuclease -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141469.06 Da        Isoelectric Point: 5.4607

>NTDB_id=434104 HCU65_RS05600 WP_219947334.1 1137379..1141083(+) (addA) [Bacillus atrophaeus strain PENSV20]
MQIPKPADSTWTDDQWNAIVSSGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLRKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPKTWLEGFVHLYDASEKSTIEELPFYPYMKEDIAMVLSAVKEKLQRA
LELTKVPGGPAPRADNFLDDLAQTEDLIAHSADFTELYKRVPAVSFKRAKAVKGDEFDQALTEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLSDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAVNESGERKPSEAAAYYQEQ
FQEVLVDEYQDTNLVQEAILQLVTSGPEASGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTETGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKVGEVDYDEQAELKLGASYPPNDQTETELLLIDRAEEKEASEETEELETVQLEAKAIAKQIR
KLVSSPYKVYDGKSKTHSNIRYRDIVILLRSMPWAPQIMEELKAQGIPVYANLTSGYFEAVEVSVAISALKVIDNPYQDI
PLASVLRSPIVGADENELSLIRMHNKKAPFYEAMKDYLSGGDRNDELYQKLNAFFELLQKWRSFSKNHSVSELIWEVYRD
SKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQASASQPDWLLPDFERYQAKTYLDFIGPALIRHRDMGTDALQGLASQADISEHPARFNIQMVSAQD
LLDDDLEERQEEKSGRLDAIRRGEPVSGSFEFEEQARQRLSWRYPYHDVTKIRTKQSVSEIKRKKEYEDEYSGRSLIRPA
DGNMLYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHAPSKEEAERIVKRLYEKELLTEDQQAVIDLEEIVQFFHTEIGE
KLIGAKWIHREVPFSMALSAQDAFLDTESKDEPILVQGIIDCLYETDEGLFLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIELYTKAVEQITKTNVKGRALYFFDGGHILNV

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=434104 HCU65_RS05600 WP_219947334.1 1137379..1141083(+) (addA) [Bacillus atrophaeus strain PENSV20]
ATGCAAATTCCTAAACCGGCAGACAGCACCTGGACAGATGACCAGTGGAACGCCATTGTCTCATCCGGACAGGATATTCT
CGTTGCAGCGGCCGCTGGATCTGGAAAAACCGCTGTGCTTGTTGAGAGAATGATCCGAAAAATTACCGCGGAGGAAAATC
CGATAGATGTGGACCGTCTTTTGGTCGTGACATTTACGAACGCCTCTGCGGCTGAAATGAAGCACCGGATCGCGGAGGCT
TTGGAGAAAGAGCTCGTGAAACGTCCCGGCTCTCTTCATATCAGGCGGCAGCTGTCTCTGTTGAACCGGGCCAGCATTTC
AACCCTTCATTCGTTCTGCTTGCAGGTGCTCAGAAAATATTATTATCTCATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGGGACGAGGTCCTTGATGAACTGTTTGAAGACGAATACGCGAAGGGTGAAAAAGCG
TTTTTCGAACTGGTGGACCGGTATACGACAGACCGGCACGACTTAGACCTTCAGCACCTCGTGAAACGGGTGTATGAGTT
TTCCCGTTCCCATCCCGATCCAAAAACGTGGCTGGAAGGTTTCGTTCATCTTTACGATGCCTCAGAAAAAAGCACGATTG
AAGAACTGCCGTTTTATCCATATATGAAAGAAGACATTGCGATGGTGCTGAGTGCTGTGAAAGAAAAGCTTCAGCGGGCG
CTTGAGCTGACAAAAGTGCCGGGCGGCCCTGCGCCGCGCGCTGATAATTTTCTGGATGATCTTGCACAGACAGAGGATCT
GATCGCACATTCGGCGGATTTCACTGAATTATACAAGCGGGTTCCCGCAGTCTCCTTTAAGCGTGCCAAAGCGGTCAAAG
GAGATGAATTTGACCAGGCGCTCACAGAAGAAGCGACGGATTTACGGAACGGCGCGAAAAAACTGCTGGAAAAACTAAAA
ACGGACTATTTCACAAGAAGCCCCGAGCAGCATCTGAAAAGCCTGTCGGATATGAAGCCTGTGATTGAAACGCTCGTCCA
GCTTGTCATCAGCTACGGAAAACGGTTTGAAGCCGCCAAACAAGAGAAATCAATTATTGATTTCTCTGACTTGGAGCATT
ACTGCTTGTCTATCCTGACAGCCGTGAATGAGTCGGGGGAGCGCAAGCCGAGTGAGGCGGCGGCTTACTATCAAGAGCAG
TTCCAAGAGGTGCTGGTTGACGAATATCAAGATACAAATCTTGTGCAGGAAGCCATACTGCAGCTTGTCACAAGCGGGCC
TGAAGCTTCAGGAAACTTGTTTATGGTAGGGGATGTCAAACAGTCCATCTACCGATTCAGGCTTGCCGAACCGCTTCTTT
TCCTTTCTAAATATAAACGATTTACCGAAACGGGAGAGGGGACAGGCCGGAAGATTGATTTGAACAAAAACTTTCGAAGC
CGGGCGGATATTTTAGACAGCACGAACTTTCTGTTTAAGCAGCTCATGGGCGGCAAGGTCGGCGAGGTTGATTATGATGA
GCAGGCGGAATTGAAGCTAGGAGCCTCTTACCCTCCGAATGACCAGACTGAAACGGAGCTGTTGCTGATTGACAGGGCGG
AGGAAAAAGAGGCGAGTGAAGAAACAGAAGAGCTCGAAACCGTTCAGCTTGAAGCAAAAGCGATTGCAAAGCAGATTCGA
AAGCTCGTATCCTCTCCTTACAAAGTCTATGATGGAAAATCGAAAACACACAGCAATATCCGCTACCGCGATATCGTGAT
TTTACTGCGTTCCATGCCGTGGGCGCCGCAAATCATGGAAGAACTGAAAGCTCAAGGCATACCCGTCTATGCCAATTTGA
CATCAGGCTATTTTGAAGCGGTCGAGGTGTCTGTTGCGATTTCCGCTTTAAAAGTGATTGATAATCCGTACCAAGATATT
CCTCTCGCTTCCGTGCTGCGCTCGCCGATTGTCGGCGCGGATGAAAATGAGCTTTCACTGATTCGGATGCATAATAAAAA
AGCGCCTTTCTATGAAGCGATGAAGGACTATCTTTCAGGCGGAGACCGAAATGACGAGCTATATCAAAAGCTGAACGCGT
TTTTTGAGCTTTTGCAAAAATGGCGCTCTTTTTCGAAAAACCATTCAGTTTCCGAGCTGATATGGGAAGTTTATCGTGAC
AGCAAATATATGGACTATGTCGGCGGTATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGTTCTCTATGACCGGGC
ACGCCAGTATGAATCGACGGCATTTCGCGGTTTATTTCGTTTCTTGCGGTTTATTGAACGTATGCAGGAACGGGGCGATG
ACCTCGGGACAGCGAGAGCGCTCAGCGAGCAGGAGGACGTGGTCCGCCTGATGACGATTCACAGCAGCAAGGGGCTTGAA
TTCCCTGTCGTATTCGCTGCGGGGCTTGGACGGAATTTTAATATGATGGATTTGAATAAATCATATCTGCTGGACAAGGA
GCTTGGTTTCGGCACAAAGTTTATTCACCCCGAATGGCGCATCAGCTATCCGACCCTTCCGCTCATCGCCATGAAGAAAA
AAATGCGCCGGGAGCTGCTGTCAGAAGAGCTGCGTGTGCTTTACGTGGCTTTAACAAGAGCGAAGGAAAAGCTGTTTCTT
GTCGGTTCCTGCAAGAATCGTGAGAAGCAGCTGGCGAAATGGCAGGCTTCAGCGTCTCAGCCAGACTGGCTTCTCCCGGA
TTTTGAGCGGTATCAGGCGAAAACGTATTTGGATTTCATCGGTCCCGCATTGATTCGTCACAGAGACATGGGGACAGATG
CTTTACAAGGGCTTGCGAGTCAAGCTGACATATCAGAACACCCTGCCCGGTTTAACATCCAAATGGTCAGCGCCCAAGAT
TTATTGGATGATGATCTGGAAGAAAGACAAGAGGAAAAGAGCGGCCGTCTTGACGCCATCCGCCGCGGTGAGCCTGTGTC
CGGTTCGTTTGAATTCGAAGAACAAGCGCGGCAACGCCTGTCATGGCGATACCCTTATCACGACGTGACAAAAATTCGCA
CGAAGCAGTCTGTTTCCGAGATCAAGAGAAAGAAAGAATACGAGGACGAATACAGCGGCCGCTCTCTTATCAGACCGGCT
GACGGAAACATGTTGTACAGGCGCCCTGCGTTTATGATGAAAAAAGGGCTGACTGCGGCTGAAAAAGGAACGGCGATGCA
CACCGTTATGCAGCATATCCCGCTGACACATGCGCCGTCAAAAGAGGAAGCGGAACGTATTGTCAAACGTCTTTATGAAA
AAGAGCTGCTCACCGAAGACCAGCAAGCCGTTATTGACCTAGAGGAAATCGTGCAATTTTTCCATACTGAAATCGGCGAA
AAGCTGATCGGCGCAAAATGGATTCACCGCGAAGTGCCGTTTAGCATGGCGCTTTCAGCACAAGATGCGTTTCTGGACAC
TGAATCTAAAGATGAGCCTATATTGGTGCAAGGAATTATTGACTGTCTGTATGAGACCGATGAAGGGCTTTTCTTGCTGG
ATTACAAAACGGACCGAATCGAAGGGAAATTCCAGCACGGATTTGAGGGGGCGGCTCCTATTCTTAAAAAACGGTACGAA
ACTCAAATCGAGCTATACACAAAAGCTGTAGAACAAATAACGAAAACCAATGTGAAGGGACGTGCACTCTACTTCTTCGA
CGGAGGACACATTTTGAACGTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

86.629

100

0.866