Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   HCU65_RS05595 Genome accession   NZ_CP050705
Coordinates   1133892..1137392 (+) Length   1166 a.a.
NCBI ID   WP_219947333.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain PENSV20     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1128892..1142392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCU65_RS05570 (HCU65_05590) - 1129311..1130798 (+) 1488 WP_373565613.1 monooxygenase -
  HCU65_RS05575 (HCU65_05595) - 1130882..1131397 (+) 516 WP_219947331.1 MarR family transcriptional regulator -
  HCU65_RS05580 (HCU65_05600) - 1131471..1132619 (-) 1149 WP_219947332.1 AbrB family transcriptional regulator -
  HCU65_RS05585 (HCU65_05605) - 1132826..1133152 (-) 327 WP_219947774.1 four-helix bundle copper-binding protein -
  HCU65_RS05590 (HCU65_05610) - 1133249..1133686 (-) 438 WP_003327683.1 ferritin family protein -
  HCU65_RS05595 (HCU65_05615) addB 1133892..1137392 (+) 3501 WP_219947333.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  HCU65_RS05600 (HCU65_05620) addA 1137379..1141083 (+) 3705 WP_219947334.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  HCU65_RS05605 (HCU65_05625) sbcD 1141154..1142332 (+) 1179 WP_106034625.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134434.26 Da        Isoelectric Point: 5.2036

>NTDB_id=434103 HCU65_RS05595 WP_219947333.1 1133892..1137392(+) (addB) [Bacillus atrophaeus strain PENSV20]
MGAEFLVGRSGSGKTKLIIDNIQDELRRNPFGKPIIFLVPDQMTFLMEYELAKTPGIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTSTGIQMLLRKLIEEHKQEFKVYQKASDKSGFTEQVERMLTEFKRYCLEPEDIRRMIESGTATDYRGERVLTD
KLHDLYILYQQMEKSLENQYLHSEDYLTLLAEQIPYAEDLKGAHVYVDGFYQFTPQEFRVLTQLMAQAEHVTFSLTADKP
SHVQAPDELDLFRMTGKTYYQLHQAASELHLDITNRELTETKRYQQTPELAHLEAMYDARPAVPFLEKQEAFTVMQAANR
RTELEGIAREIHALVRDKGLRYKDMAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLVEFIRSSLDVIKGNWRY
EAVFRCVKTELLFPLDRPKNIIREQVDQLENYCIAYGIKGDRWTRGDRFVYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFRLQKRMKQAKTVQDMAEALYLYLEETDIPLKLDQERQRAEEDGRIIEAQQHNQAWDAVIQLLEEFVEMMGEDEI
SLSLFQQMMETGAESLKFALIPPALDQVFVGNMDLSRMYGTRCTFVIGANDGVLPARPEDDGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSTVVKRLTELFPEHQERLLTNEPEQVSDCEQLLYLVNKS
VAQSYTASQFRLWLKEYDISDVWWSAYNVLMSEQDRLNSKKLFSSLFFRNEVKPLERAVSRQLYGEHIHGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKDQCNKFSYEAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVARVSGILSEHAKASGFAPVGLELGFGGKGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLSEVYYGLALQMLTYLDLSITYSADWLGMKATPAGVLYFHIHDPMIQASVPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEEEFHLLTQHVRRTFQEAGEQITDGRVSIEPYKLKDKTP
CTYCSFKSVCQFDESLEENEYRTLKAEKDHTILDWLKKEGDGDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=434103 HCU65_RS05595 WP_219947333.1 1133892..1137392(+) (addB) [Bacillus atrophaeus strain PENSV20]
TTGGGAGCAGAGTTTTTAGTAGGCAGATCCGGGAGTGGAAAAACGAAGCTGATCATAGACAACATTCAAGATGAGCTGCG
CCGGAATCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCTGGGATAGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGTTTGGCCTGGCGTGTCCTGCAGCATACGGGC
GGAATGAACCGGCCGTTTGTCACCAGCACGGGAATCCAAATGCTTTTGAGAAAGCTCATTGAAGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGAACAGGTAGAACGGATGCTGACGGAATTTAAACGGTACT
GCCTTGAGCCTGAAGATATCCGCCGCATGATTGAAAGCGGAACTGCCACAGACTATCGCGGCGAGCGGGTGTTAACTGAT
AAGCTGCATGATTTGTATATTTTATATCAGCAGATGGAAAAGAGCCTTGAAAACCAGTATCTTCATTCAGAGGATTATTT
AACGCTGCTGGCGGAACAGATCCCGTATGCGGAAGATTTGAAAGGGGCGCATGTCTATGTAGACGGCTTTTATCAGTTTA
CTCCGCAGGAATTCAGAGTGCTTACGCAGCTTATGGCACAGGCTGAGCATGTGACGTTTTCACTGACGGCGGACAAGCCG
TCCCATGTGCAGGCGCCTGATGAGCTGGATCTGTTCAGAATGACAGGCAAAACATATTATCAGCTCCATCAAGCAGCTTC
GGAGCTTCATTTGGATATCACAAACAGAGAACTGACCGAGACGAAACGATATCAGCAAACACCGGAGCTTGCCCATCTAG
AAGCAATGTATGACGCGCGCCCGGCTGTTCCGTTTTTGGAAAAGCAAGAAGCGTTCACCGTGATGCAGGCTGCGAACAGG
CGGACAGAGCTGGAAGGAATTGCCCGCGAAATTCATGCTCTTGTAAGAGATAAGGGATTACGCTACAAGGATATGGCAAT
TCTCGCCCGCCAGCCGGAAGATTATAAGGATATGGTCAAAGAAGTATTTGCAGATTATGAAATTCCTTACTTTATAGATG
GAAAGGCATCCATGCTGAACCATCCGCTTGTTGAGTTTATCCGTTCAAGCCTTGATGTCATAAAGGGAAATTGGAGATAT
GAAGCGGTGTTTCGCTGTGTGAAAACCGAACTGTTATTCCCGCTTGACCGTCCGAAGAACATCATAAGGGAGCAGGTCGA
TCAACTGGAAAACTATTGTATTGCTTACGGAATTAAAGGCGACCGATGGACGAGAGGCGACCGTTTTGTTTACAGGCGGT
TTGTTTCACTGGATGATGATTTTGCGCAGACAGATCAAGAGATTGAAATGGAAAACATGTTGAATGACACAAGAGACTGG
ATTGTTCCGCCGCTTTTCCGTCTTCAAAAACGGATGAAACAAGCAAAAACGGTCCAAGACATGGCGGAGGCTCTTTATCT
TTATTTAGAAGAAACGGATATTCCGTTAAAGCTGGACCAAGAAAGACAGCGCGCGGAAGAGGACGGGAGAATTATCGAAG
CGCAGCAGCATAATCAGGCTTGGGACGCTGTCATACAGCTTCTCGAGGAATTTGTGGAAATGATGGGCGAAGACGAAATT
TCTCTGTCTCTTTTTCAGCAAATGATGGAGACGGGTGCGGAATCGTTAAAATTCGCTCTGATTCCGCCTGCACTCGATCA
AGTCTTTGTCGGCAATATGGATTTATCGAGAATGTACGGTACACGCTGCACCTTTGTTATCGGTGCGAACGACGGCGTAC
TTCCGGCGCGGCCTGAGGATGACGGGGTGCTGTCTGATGACGACCGGGAATGGCTGAAAACCATTGGAGTCGAGCTGTCA
TCCGGCGGGCGCGAACGTCTGCTTGATGAGCACTTTCTCATATATATGGCGCTTTCCAGTCCGTCTGACCGCCTGTATGT
ATCCTACCCGATCGCAGACGCCGAAGGTAAAACGCTTTTGCCGTCAACTGTGGTCAAACGGCTTACAGAACTGTTTCCGG
AGCATCAGGAAAGGCTTTTGACCAATGAACCGGAACAGGTCAGCGACTGCGAACAGCTCTTGTATCTCGTCAATAAAAGC
GTGGCGCAATCGTATACGGCAAGCCAATTCAGGTTGTGGTTAAAGGAGTATGACATCAGTGATGTATGGTGGAGCGCGTA
TAATGTATTGATGAGTGAACAGGACAGGCTGAACTCGAAAAAACTTTTCTCCAGCCTGTTTTTCAGAAATGAAGTCAAAC
CGCTTGAACGCGCTGTATCGAGACAGCTGTACGGCGAGCATATTCATGGAAGTGTGTCCAGAATGGAGACCTTTAACGCT
TGTCCGTTCTCCCATTTCGCGTCCCACGGCTTACATCTGAAAGAACGGCAATTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTCCATTCCAGCCTGAAGCTGATTTCAGACCGGCTTCGTGAACAAAAGCTGGATTGGCGCGATTTAACAAAAG
ACCAATGTAACAAGTTCTCTTACGAGGCGGTGGAGCGGCTCGCGCCGAAGCTCCAAAAAGAAATTCTGCTTAGCTCAAAC
AGACATTATTATGTCAAGGAAAAGCTGCAAAAAATTGTGGCGCGCGTGTCCGGCATTTTAAGCGAGCATGCGAAGGCGAG
CGGGTTTGCACCGGTTGGGCTTGAGCTTGGTTTTGGCGGAAAAGGCCCGCTGCCGCCTCTTACATTCACATTAAAAAACG
GCTGCACAATGGAGCTTGTCGGACGCATTGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGGCTGCTGCTGAGAATAGTG
GATTATAAATCCAGCGACAAGGGTCTTGATTTGTCAGAGGTATATTACGGCCTTGCCCTGCAAATGCTCACGTACCTTGA
TTTATCCATTACGTATTCGGCAGATTGGCTCGGCATGAAGGCTACACCGGCCGGCGTTCTTTATTTCCATATTCACGACC
CGATGATTCAAGCCAGCGTTCCGCTCGGGCTTGATGAAATAGAGCAGGAAATTTTTAAGAAATTTAAGATGAAAGGCCTG
CTGCTCGGCGATCAGGAAGCGATTCGATTGATGGATACGACGCTCCAAGAGGGAAGGTCAAATATCATTAATGCCGGGCT
TAAAAAAGACGGTTCATTAAGGTCTGATTCAGCGGCGGTCGGAGAAGAGGAATTTCATCTGCTGACACAGCACGTGCGGC
GGACCTTTCAGGAGGCCGGCGAACAAATCACCGACGGGCGTGTGTCGATTGAACCGTATAAGCTGAAAGATAAAACACCC
TGCACATACTGCTCATTCAAATCAGTATGCCAGTTCGATGAATCATTGGAAGAAAACGAATATCGCACGCTGAAAGCGGA
AAAGGATCATACCATATTAGATTGGCTGAAAAAGGAGGGAGATGGCGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

88.851

100

0.889