Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HCU65_RS00580 Genome accession   NZ_CP050705
Coordinates   105937..107316 (+) Length   459 a.a.
NCBI ID   WP_219946955.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain PENSV20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100937..112316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCU65_RS00560 (HCU65_00560) ctsR 101291..101755 (+) 465 WP_003328320.1 transcriptional regulator CtsR -
  HCU65_RS00565 (HCU65_00565) - 101769..102326 (+) 558 WP_003328319.1 UvrB/UvrC motif-containing protein -
  HCU65_RS00570 (HCU65_00570) - 102326..103417 (+) 1092 WP_010787416.1 protein arginine kinase -
  HCU65_RS00575 (HCU65_00575) clpC 103414..105852 (+) 2439 WP_003328317.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  HCU65_RS00580 (HCU65_00580) radA 105937..107316 (+) 1380 WP_219946955.1 DNA repair protein RadA Machinery gene
  HCU65_RS00585 (HCU65_00585) disA 107320..108402 (+) 1083 WP_010787418.1 DNA integrity scanning diadenylate cyclase DisA -
  HCU65_RS00590 (HCU65_00590) - 108523..109623 (+) 1101 WP_003328314.1 PIN/TRAM domain-containing protein -
  HCU65_RS00595 (HCU65_00595) ispD 109637..110335 (+) 699 WP_219946956.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  HCU65_RS00600 (HCU65_00600) ispF 110328..110804 (+) 477 WP_003328312.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49636.03 Da        Isoelectric Point: 7.9893

>NTDB_id=434089 HCU65_RS00580 WP_219946955.1 105937..107316(+) (radA) [Bacillus atrophaeus strain PENSV20]
MAKSKTKFICQSCGYESAKWMGKCPGCGAWNTMTEETVKKTPANRRAAFAHSTQTVKKPSPITSIETSEEPRIKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSTHSVLYISGEESVKQTKLRADRLDINNQTLHVLAETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVSVSIASSFRDTPPNPSDCIIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=434089 HCU65_RS00580 WP_219946955.1 105937..107316(+) (radA) [Bacillus atrophaeus strain PENSV20]
ATGGCTAAATCAAAAACGAAATTTATCTGCCAATCCTGCGGCTATGAATCCGCAAAGTGGATGGGGAAATGTCCAGGCTG
CGGAGCTTGGAACACAATGACAGAAGAAACAGTAAAAAAAACACCGGCTAATCGCAGAGCAGCTTTTGCACACTCTACCC
AAACCGTAAAGAAACCTTCACCTATCACATCAATAGAAACATCTGAAGAACCCCGAATTAAAACACAGCTTGGAGAATTT
AACAGAGTGCTGGGCGGCGGTGTTGTGAAGGGCTCACTTGTCTTAATTGGAGGAGATCCCGGTATTGGTAAGTCAACCTT
GCTGCTTCAGGTATCCGCTCAGTTATCAGACTCCACGCATAGTGTGCTCTACATTTCAGGCGAGGAATCTGTGAAACAAA
CGAAACTCAGAGCAGATCGGCTCGATATTAATAACCAAACGTTACATGTTTTAGCTGAAACCGACATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCATTTGTGGTTGTTGACTCCATTCAGACGGTCTATCAAAGCGATATTACATCAGC
TCCGGGCAGTGTGTCGCAAGTCAGGGAGTGTACGGCAGAGCTGATGAGAATTGCCAAGACAAAGGGTATACCGATTTTTA
TCGTAGGACATGTAACAAAAGAAGGTTCAATTGCTGGTCCGAGACTGTTGGAACATATGGTTGATACTGTTCTTTACTTT
GAAGGAGAGCGGCATCATACGTTCCGCATCCTGCGGGCAGTAAAAAACCGTTTCGGCTCTACAAATGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGTCTGACAGAGGTGCTGAATCCTTCTGAAATCTTTCTTGAGGAACGATCAGCCGGAGCGGCAG
GTTCCAGCATAGTAGCATCTATGGAAGGCACCAGACCGATTCTCGTGGAAATACAGGCCCTTATCTCACCGACCAGCTTT
GGAAATCCCCGGCGTATGGCCACGGGAATTGACCATAACAGAGTATCACTGCTCATGGCTGTGTTAGAAAAAAGAGTCGG
GCTGCTGCTTCAAAATCAAGATGCGTATTTAAAGGTAGCAGGCGGCGTTAAGCTGGATGAACCGGCAATCGATCTCGCTG
TATCTGTCAGTATTGCATCAAGCTTTAGAGATACACCGCCAAATCCTTCCGACTGCATTATAGGAGAAGTCGGTTTAACA
GGTGAGGTCCGCAGGGTATCCAGAATTGAACAGCGTGTGAAAGAAGCTGCTAAACTTGGATTTAAGCGTATGATCATACC
TGCGGCGAATTTAGATGGATGGACAAAACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.886

99.782

0.937
  radA Streptococcus mitis NCTC 12261

63.135

98.693

0.623
  radA Streptococcus pneumoniae Rx1

63.135

98.693

0.623
  radA Streptococcus pneumoniae D39

63.135

98.693

0.623
  radA Streptococcus pneumoniae R6

63.135

98.693

0.623
  radA Streptococcus pneumoniae TIGR4

63.135

98.693

0.623
  radA Streptococcus mitis SK321

62.914

98.693

0.621