Detailed information NTG0432859

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: G4G28_RS05395
• Genome accession: NZ_CP050454
• Coordinates: 1164977..1165888 (-)
• Length: 303 a.a.
• NCBI ID: WP_183108114.1
• Uniprot ID: A0A7G5ZBI1
• Organism: Massilia sp. Dwa41.01b
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1159977..1170888

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G4G28_RS05365 (G4G28_05360)	-	1160921..1161331 (+)	411	WP_182991147.1	NUDIX domain-containing protein	-
G4G28_RS05370 (G4G28_05365)	-	1161364..1162665 (+)	1302	WP_182991148.1	D-amino acid dehydrogenase	-
G4G28_RS05375 (G4G28_05370)	yidD	1162845..1163327 (-)	483	WP_182991149.1	membrane protein insertion efficiency factor YidD	-
G4G28_RS05380 (G4G28_05375)	yacG	1163330..1163518 (-)	189	WP_182991150.1	DNA gyrase inhibitor YacG	-
G4G28_RS05385 (G4G28_05380)	zapD	1163549..1164304 (-)	756	WP_182991151.1	cell division protein ZapD	-
G4G28_RS05390 (G4G28_05385)	coaE	1164369..1165019 (-)	651	WP_183108113.1	dephospho-CoA kinase	-
G4G28_RS05395 (G4G28_05390)	pilD	1164977..1165888 (-)	912	WP_183108114.1	A24 family peptidase	Machinery gene
G4G28_RS05400 (G4G28_05395)	-	1166220..1166894 (+)	675	WP_182991154.1	hypothetical protein	-
G4G28_RS05405 (G4G28_05400)	-	1166887..1167714 (+)	828	WP_182991155.1	RES family NAD+ phosphorylase	-
G4G28_RS05410 (G4G28_05405)	-	1167821..1168996 (+)	1176	Protein_1113	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
G4G28_RS05415 (G4G28_05410)	-	1169531..1170433 (+)	903	WP_182991157.1	phospholipase D family protein	-

Sequence

Protein

• Length: 303 a.a.
• Molecular weight: 32769.86 Da
• Isoelectric Point: 8.3284

>NTDB_id=432859 G4G28_RS05395 WP_183108114.1 1164977..1165888(-) (pilD) [Massilia sp. Dwa41.01b]
METLLFADPASFGPTLVAGLFGLLIGSFLNVVIHRVPKMMQRESDNYVAHESGKELPHTDRYNLMVPRSACPHCGHQISA
LENIPVVSWLALRGKCRNCKAPISPRYPAVELLTGILSALLVWTFGSGIAGLATLLFLYLLIAMTFIDFDTQLLPDDLTY
PLLWAGLLVNLNGTFVPLRDAVIGAAAGYLVLWSVYWIFKLVRGKEGMGYGDFKLLAALGAWLGWQMLPAIILLSSVVGA
VVGISLIVFARRGRDNPIPFGPYLAAAGLIALLYGPQLTAGLQQFPGGLTCPLRPLPPLFPSA

Nucleotide

• Length: 912 bp

>NTDB_id=432859 G4G28_RS05395 WP_183108114.1 1164977..1165888(-) (pilD) [Massilia sp. Dwa41.01b]
TTGGAAACTCTTCTCTTCGCCGACCCGGCATCGTTTGGCCCTACCCTGGTGGCCGGCCTGTTCGGCCTCCTGATCGGCAG
CTTCCTGAACGTGGTGATCCACCGCGTGCCGAAGATGATGCAGCGCGAATCGGACAATTATGTCGCCCACGAGAGCGGCA
AGGAATTGCCGCACACGGACCGCTACAACCTGATGGTGCCACGCTCGGCCTGCCCGCACTGCGGCCACCAGATCAGCGCG
CTCGAGAACATTCCCGTGGTCAGCTGGCTGGCGCTGCGCGGCAAATGCCGCAACTGCAAGGCGCCGATCTCGCCGCGCTA
CCCGGCCGTCGAACTGCTGACCGGGATACTGTCGGCCCTGCTGGTCTGGACCTTCGGCAGCGGCATCGCCGGCCTGGCGA
CGCTGCTGTTCCTCTACCTGCTCATTGCGATGACCTTCATCGACTTCGACACCCAGCTGCTGCCCGACGACCTCACCTAC
CCGCTGCTGTGGGCGGGCCTGCTGGTCAACCTGAACGGGACCTTCGTGCCGCTGCGCGACGCCGTCATTGGCGCGGCCGC
CGGCTACCTGGTGCTGTGGAGCGTGTACTGGATCTTCAAGCTGGTGCGCGGCAAGGAAGGCATGGGCTATGGCGACTTCA
AGCTGCTGGCCGCGCTCGGCGCCTGGCTCGGCTGGCAGATGCTGCCGGCCATCATCCTGCTGTCCTCGGTGGTGGGCGCT
GTCGTCGGCATCAGCCTGATCGTGTTCGCTCGCCGCGGCCGCGACAATCCGATCCCGTTCGGGCCCTACCTGGCAGCCGC
CGGCCTGATCGCCCTGCTCTACGGGCCGCAACTGACGGCCGGCCTGCAGCAGTTCCCTGGCGGCCTGACATGCCCCCTGC
GACCGCTCCCACCGCTTTTTCCGTCGGCCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

3D structure

• AlphaFold DB: A0A7G5ZBI1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	58.052	88.119	0.512
pilD	Vibrio campbellii strain DS40M4	55.682	87.129	0.485
pilD	Acinetobacter baumannii D1279779	50.943	87.459	0.446
pilD	Acinetobacter nosocomialis M2	49.811	87.459	0.436
pilD	Neisseria gonorrhoeae MS11	50	85.149	0.426