Detailed information NTG0432336

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HBN34_RS21040
• Genome accession: NZ_CP050403
• Coordinates: 3776286..3777146 (+)
• Length: 286 a.a.
• NCBI ID: WP_001152277.1
• Uniprot ID: A0A009HWK1
• Organism: Acinetobacter baumannii strain VB2486
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3771286..3782146

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HBN34_RS18225 (HBN34_18225)	secG	3771891..3772220 (-)	330	WP_000555570.1	preprotein translocase subunit SecG	-
HBN34_RS18230 (HBN34_18230)	tpiA	3772233..3773027 (-)	795	WP_000016931.1	triose-phosphate isomerase	-
HBN34_RS18235 (HBN34_18235)	pilB	3773318..3775030 (+)	1713	WP_001274987.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HBN34_RS18240 (HBN34_18240)	pilC	3775060..3776286 (+)	1227	WP_000279216.1	type II secretion system F family protein	Machinery gene
HBN34_RS21040 (HBN34_18245)	pilD	3776286..3777146 (+)	861	WP_001152277.1	prepilin peptidase	Machinery gene
HBN34_RS18250 (HBN34_18250)	coaE	3777148..3777744 (+)	597	WP_000874125.1	dephospho-CoA kinase	-
HBN34_RS18255 (HBN34_18255)	-	3777741..3778655 (-)	915	WP_000075011.1	DMT family transporter	-
HBN34_RS18260 (HBN34_18260)	rlmB	3778691..3779440 (-)	750	WP_001118150.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
HBN34_RS18265 (HBN34_18265)	-	3779542..3779868 (-)	327	WP_000099685.1	pyrimidine/purine nucleoside phosphorylase	-
HBN34_RS18270 (HBN34_18270)	-	3779945..3781255 (-)	1311	WP_000753277.1	tetratricopeptide repeat protein	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32038.43 Da
• Isoelectric Point: 7.1041

>NTDB_id=432336 HBN34_RS21040 WP_001152277.1 3776286..3777146(+) (pilD) [Acinetobacter baumannii strain VB2486]
MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACQQP
IRWYQNIPVISWLVLRGKCGHCQHPISIRYPAIELLTMLCSLVVVIVFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=432336 HBN34_RS21040 WP_001152277.1 3776286..3777146(+) (pilD) [Acinetobacter baumannii strain VB2486]
ATGCAAGACATCATTGCGTATTTTATTCAAAACTTAACTGCACTTTATATTGCTGTTGCACTCGTGAGCCTATGTATCGG
TAGCTTTCTTAATGTAGTGATTTACCGCACGCCAAAAATGATGGAGCAAGATTGGCAGCAAGAATGCCAAATGCTGCTCA
ATCCTGAGCAACCCATTATTGATCATGAGAAGTTAACTTTAAGTAAGCCTGCTTCATCGTGCCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCTGTTATAAGCTGGCTTGTGTTAAGAGGAAAATGTGGTCATTGCCAGCACCCGATTAG
TATTCGCTATCCAGCCATCGAACTACTCACTATGTTATGTTCATTAGTAGTCGTCATAGTGTTTGGTCCAACCATACAAA
TGCTTTTTGGACTCGTCCTCACATGGGTACTGATAGCCCTTACCTTTATTGATTTCGATACACAATTATTGCCTGATCGC
TTTACCCTACCTTTAGCTGCGCTCGGCTTAGGTATTAATACCTTTAATATTTATACCTCACCCAACTCAGCCATTTGGGG
TTATCTCATTGGTTTCCTATGTCTTTGGATTGTCTATTACTTATTTAAAGTGATCACTGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCAGCATTAGGAGCATGGATGGGGCCATTGATGCTGCCGTTAATTGTGTTATTGTCATCGTTA
CTTGGCGCAATCATTGGCATCATTTTATTAAAATTAAGAAATGACAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT
TGCTGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-127)

3D structure

• AlphaFold DB: A0A009HWK1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	98.951	100	0.99
pilD	Acinetobacter nosocomialis M2	98.601	100	0.986
pilD	Vibrio cholerae strain A1552	54.044	95.105	0.514
pilD	Vibrio campbellii strain DS40M4	52.768	94.755	0.5
pilD	Neisseria gonorrhoeae MS11	47.727	92.308	0.441