Detailed information NTG0431984

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HB636_RS22980
• Genome accession: NZ_CP050360
• Coordinates: 4598760..4599560 (-)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain 47733
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4593760..4604560

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB636_RS22960 (HB636_22975)	dksA	4594340..4594795 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
HB636_RS22965 (HB636_22980)	sfsA	4594958..4595674 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HB636_RS22970 (HB636_22985)	thpR	4595674..4596210 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
HB636_RS22975 (HB636_22990)	hrpB	4596283..4598712 (+)	2430	WP_032433845.1	ATP-dependent helicase HrpB	-
HB636_RS22980 (HB636_22995)	pilD	4598760..4599560 (-)	801	WP_004191960.1	A24 family peptidase	Machinery gene
HB636_RS22985 (HB636_23000)	-	4599557..4600303 (-)	747	WP_004145897.1	type II secretion system protein N	-
HB636_RS22990 (HB636_23005)	-	4600293..4600778 (-)	486	WP_004145896.1	type II secretion system protein M	-
HB636_RS22995 (HB636_23010)	gspL	4600771..4601964 (-)	1194	WP_032416685.1	type II secretion system protein GspL	-
HB636_RS23000 (HB636_23015)	gspK	4601951..4602937 (-)	987	WP_077255453.1	type II secretion system minor pseudopilin GspK	-
HB636_RS23005 (HB636_23020)	gspJ	4602934..4603530 (-)	597	WP_032433846.1	type II secretion system minor pseudopilin GspJ	-
HB636_RS23010 (HB636_23025)	gspI	4603527..4603892 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HB636_RS23015 (HB636_23030)	gspH	4603889..4604404 (-)	516	WP_032422016.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=431984 HB636_RS22980 WP_004191960.1 4598760..4599560(-) (pilD) [Klebsiella pneumoniae strain 47733]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=431984 HB636_RS22980 WP_004191960.1 4598760..4599560(-) (pilD) [Klebsiella pneumoniae strain 47733]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421